清酒火落菌の栄養物質と其の拮抗物質に関する研究(第10報) : 米蛋白貭分解物の発育促進性

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Rice proteins, i.e. albumin, globulin, prolamin and glutelin were fractionated and purified from the rice for Sake-brewing. The proteins were hydrolyzed by conc. hydrochloric acid, pancreatin, or Taka-protease isolated from Taka-diastase. when these partial hydrolyzates were added to the complete synthetic medium for Hiochi-kin, they showed a considerable promoting effect on the growth of strain T107. On the contary, complete hydrolyzate did not show such a promoting effect.Hydrolyzates of rice glutelin were useful nitrogen-source of synthetic medium for Hiochi-kin. Partial hydrolyzates by hydrochloric acid (6N-HCl, 100℃, 1 hr. and 12N-HCl, 37℃, 20 hrs.) and hydrolyzates by Taka-protease (37℃, 120 hrs.) were better than the mixture of amino acids (control) as the N-source of synthetic medium.In the processes of Sake-brewing, hydrolyzed components of rice proteins may be the active factors for the growth of Hiochi-kin, and the activities may be influenced by the degree of decomposition of the proteins.
社団法人日本生物工学会の論文
1957-01-15