食品中の核酸成分に関する研究 : (第2報)食用キノコにおける5'-ヌクレオチドの分布

概要

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Fractions of 5'-nucleotides in boiled extract of 10 kinds of mushrooms, i.e., Psalliota bisporus, Lentinus edodes (Shii-take), Tricholoma matsutake (Matsutake) etc. were separated and analysed by means of ion exchange chromatography on a Dowex1X8 column (formate form). Four fractions of raw Lentinus edodes were identified as 5'-cytidylic acid (5'-CMP), 5'5'-adenylic acid (5'AMP), 5'-uridylic acid (5'UMP) and 5'-guanylic acid (5'-GMP), and two of P. bisporus as 5'-AMP and 5'-UMP, 5'-GMP, which is called "a tasty component of dried Shii-take" was also found in boiled extracts of Lyophyllum aggregatum (amounted to 0.15% on dry basis), Lactarius hatsudake, and T. matsutake, etc. The level of 5'-nucleotides of dried L. edodes extracted with boiling water was higher than that of 5'-nucleotides extracted with cold perchloric acid or sulfuric acid. The amount of individual 5'-nucleotide of dried L. edodes was about two times as much as that of 5'-nucleotide of the raw sample, on a dry basis.The amount of 5'-nucleotides in some mushrooms was fairly more than found in vegetables. The amount of free 5'-nucleotide in mushrooms was no more than about one tenth of the amount of ribonucleic acid in them.
社団法人日本生物工学会の論文
1964-07-15