グルタミン酸の醗酵生産とその応用に関する研究 (第3報) : 溶存酸素濃度の影響

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The effects of the concentration of dissolved oxygen on the respiration rate, growth rate and the rate of glutamic acid formation were examined using strain No. 532-2 as test organism.Applying the equation of MICHAELIS-MENTEN to the relation between respiration rate and the concentration of dissolved oxygen, MICHAELIS constant Km was estimated to be 0.97×10^<-5>M. Variation in the value of l/Km, which is considered to represent the affinity of the test organism to oxygen, with the change of medium pH may give one reason for fall in respiration rate in acidic side. In the case where cell concentration was very high and the distribution of dissolved oxygen might not be homogeneous, the critical concentratino of dissolved oxygen for respiration was varied superficially, while the actual value was in the range of 1.0〜2.0×10^<-6>M.The relation between specific growth rate (μ) and the dissolved oxygen concentration ([O_2]) was found to obey the equation, μ=C[O_2]^κ, within the range from 10^<-4> to 10^<-7>M.The rate of glutamic acid formation accompanied with growth was presumed to be maximum at a dissolved oxygen concentration of 10^<-5> M.
1961-09-15