B. polymyxa 1変株の生成する細菌多糖類, ラムノガラクタンの研究(第12報) : 過沃素酸酸化

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The periodate oxidation method was applied to the oxidation of rhamnogalactans different in molecular size for the structural analysis.It was found that the selective oxidation was accompolished by using an amount of sodium metaperiodate in excess of the theoretical at 10℃ for 96 hr, while in the reaction at 25℃, the occurrence of over-oxidation involving the hydrolysis of glycosidic linkages was presumed from the results of the viscosity measurements and determination of both the consumed periodate and the produced formic acid.In the normal oxidation, 0.41 mol of periodate on the average was consumed for the C_3-sugar unit with liberation of 0.17 mol of formic acid, and both rhamnose and galactose were isolated from the oxidation product at the end of the reaction.These results suggest that the reaction should occur only on the nonreducing terminal units and give support to the previous methylation study (this J., in press -1958-) that the polysaccharide possesses a branched structure and the main chains are connected with 1 : 3 glycosidic linkage.From the end group assay, the average repeating unit of the polysaccharide was calculated to be nearly 6.
社団法人日本生物工学会の論文
1958-07-15