清酒火落菌の栄養物質と其の拮抗物質に関する研究 : (第14報)葉酸拮抗物質に依る発育の阻害と清酒防腐への応用

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4-amino-folic acid (AFA), 4-amino-N^<10>-methyl-folic acid (AMFA), pteroyl-aspartic acid (PAA) and four substituted 2,4-diamino-pyrimidines were examined on their anti-folic acid activity on Hiochi-kin in the synthetic medium in which the concentration of folic acid (FA) was limited.When FA was 0.005 μg/ml, AFA and AMFA inhibited the growth of Hiochi-kin at a concentraiton of 0.0005 and 0.00005 μg/ml, respectively. PAA was not inhibitive. Among four 2,4-diamino-pyrimidines, 5-chlorophenoxy derivative was most potent, it inhibited the growth of Hiochi-kin at a concentration of 10 μg/ml. The inhibition by low concentrations of AFA was reversed by FA or Leucovorin, but that by 0.00025 μg/ml AMFA was not reversed by 0.625 μg/ml FA or 0.125 μg/ml Leucovorin. The inhibition by 20 μg/ml 2,4-diamino-5-chlorophenoxy-pyrimidine was reversed by Leucovorin, but not by FA.In watered Sake, in which any other preservative was not contained, AMFA in a concentration of 0.00025 μg/ml inhibited the growth of Hiochi-kin at 30℃ for 21 days. The amount, 0.00025 μg/ml, responses to 1/1,000,000 of salycilic acid (250 g/ml) which is generally used for the preservation of Sake. Thus AFMA may be the potent preservative for Japanese Sake.
社団法人日本生物工学会の論文
1958-11-15