グルタミン酸の醗酵生産とその応用に関する研究 : (第9報) グルタミン酸醗酵における抗生物質の効果 : (2)ペニシリンとビオチンの効果

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Glutamic acid is produced in only small quantities in a medium which contains an excess amount of biotin. However penicillin and several other antibiotics stimulated the productin of glutamic acid even in a biotin-excess media; for example, in the case where cane molasses was used as the carbon source. In this report, the effects of penicillin on the ability of producing glutamic acid and on the content of free glutamic acid within the cells of Brevibacterium sp. were investigated under comparitive conditions where the amount of biotin was either normal or in excess.Strain No. 425-40 belongs to Brevibacterium sp. was employed as the test organism. Glutamic acid was determined using decarboxylase from E. coli.The specific rate of glutamic acid production (ΔG;mg glutamic acid/mg cell. hr.) of cells harvested from normal-biotin medium (designated as A-cell) was higher than that of cells from excess-biotin (biotin 20μg/1 was added) medium (designated as B-cell). When A- and B-cell were incubated with 3.3-833 unit/ml of penicillin for 4 hours, the ΔG of each cell during incubation under the presence of penicilin was higher than the ΔG under the abence of penicillin. The ΔG of A-cell became higher when biotin was added during incubation at the concentrations of 5-100μg/1 under the presence of penicilin. In the case of A-cell, free glutamic acid in the cells was increased to the range of 24-55μg/mg cell according to the addition of biotin, but it was reduced to that of 1.5-3.5μg/mg cell by the addition of penicillin. The ratio of the external and internal amounts of glutamic acid of the cells, was 4-70 under absence of penicillin, and 250-700 when penicillin was present. In the case of B-cell, the amount of free glutamic acid in the cell and the ratios of external and internal amounts were respectively, 40-50μg/mg cell and 1-2 with no presence of penicillin, and 2-4μg/mg cell and 100-150 under the presence of penicillin.
社団法人日本生物工学会の論文
1964-02-15

グルタミン酸の醗酵生産とその応用に関する研究 : (第9報) グルタミン酸醗酵における抗生物質の効果 : (2)ペニシリンとビオチンの効果

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