食品中の核酸成分に関する研究 : (第16報)シイタケの乾燥中における核酸成分の消長

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Shii-take (Lentinus edodes) was dried with circulating heated air at 60,80 or 100℃. Nucleotides and related substances were collected by an extraction with cold perchloric acid and their amounts were determined with ion exchange chromatography and phosphatase assay. RNA and acid-soluble portions were fractionated by Schmidt-Thannhauser's method.During the drying of Shii-take at 60℃, 2.4 μmole/g dry wt. of ATP originally existed decreased to a trace amount with an increasing of ADP, and RNA was decomposed with an accumulation of 5'-nucleotides. Under drying at 100℃, a half of the original amount of ATP remained and the ADP and 5'-nucleotide contents changed only a little. When dried Shii-take was dipped in water at 70℃ or 30℃ for 30 min., decreases of RNA and ATP and increases of four kinds of 5'-nucleotides were found in restored Shii-take. About a half of each activity of RNase, PDase and PMase remained in dried samples. A crude enzyme solution of dried Shii-take formed adenosine, AMP and ADP from the standard ATP, and a mixture of 5'-nucleotides from yeast RNA, respectively. It seems reasonable to consider that the accumulation of nucleotides during the drying of Shii-take and its dipping in water is due to the action of enzymes degrading nucleic acid and related substances.
社団法人日本生物工学会の論文
1969-02-25