醗酵醪中の酢酸の迅速定量法に関する研究 : (第1報) シリカゲルによる分配クロマトグラフィー

概要

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Fermented mash generally contains a small amount of acetic acid and large amounts of virous substances, such as carbohydrate, alcohols, and nitrogenous compounds. Acoordlingly, either ion exchange or etheric extraction is carried out as the preliminary procedure to remove these substances for the determination of acetic acid content in fermented mash by means of partition chromatography on silicinc acid column. In this report, the authors has studied on the method of dettermining the acetic acid content in the samples by chromatography without any preliminary treatment. Moto (seed mash) and Moromi (mash) for the sake brewing were used as sample.By Bulen's method commonly used, silicic acid chromatograophy was carried out.Acetic acid content in Moto and Moromi was determined according to the treatments shown in Table 1,without any preliminary treatment. The recovery rate of 95-96% was observed if the additional samples contained more than 0.3mg of acetic acid.It was impossible to separate acetic acid from propionic acid when the samples contained more than 5% of ethanol. It is necessary, therefore, to keep to level of ethanol under 5% in the samples.
社団法人日本生物工学会の論文
1969-08-25