B. polymyxa 1変株の生成する細菌多糖類, ラムノガラクタンの研究(第11報) : Methylated Rhamnogalactanのメチル糖成分について

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The methylated rhamnogalactan (CH_3O : 38.28%) prepared from acetate was hydrolyzed by NH_2SO_4 after methanolysis.The following methyl sugars were characterized as free sugars and some derivatives, such as anilides, after quantitative separation from the hydrolysate by cellulose column chromatography (developer, mix. of n-butanol and petroleum benzine, 4 : 6) : 2,3,4-tri-o-methyl-l-rhamnose, 12.75; 2,4-di-o-methyl-l-rhamnose, 43.63; 2,4,6-tri-o-methyl-d-galactose, 26.80; mono-methyl-l-rhamnose, 9.21 and di-methyl-d-galactose, 7.57 (as molar per cent).Although the last-mentioned two sugars are not yet identified, the above results show that rhamnogalactan possesses a branched structure in which nonreducing terminals are composed of l-rhamnose and that the principal glycosidic linkage is 1 : 3 of rhamnose- and galactose residues. Furthermore, the portion of tri-methyl-rhamnose corresponds to an average length of ca. 8 sugar units.Although the configuration of anomeric carbon atoms is not yet established by infra-red spectrum, the changes of optical rotation to the positive direction on hydrolysis of both the unsubstituted-and methylated polysaccharides suggest that the configuration is in β-glycosidic linkage.
社団法人日本生物工学会の論文
1958-07-15