Bacillus polymyxa1変株の生成するBacterial Polysaccharideの研究(第7報) : タンク培養に依る生産(其の1)

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The polysaccharide has hitherto been produced by shaking flask culture. In the present investigation, preliminary studies concerning the production by submerged culture have been made with a laboratory fermenter (101 cont.). The results were as follows : 1) When 101 of a medium (pH 7.0) consisting of lactose, yeast-extract, inorganic nutrients and CaCO_3 were fermented by B. polymyxa var. lactoviscosus under appropriate aeration (201 per min.) and agitation (120 r.p.m.), the bacterial growth proceeded, and the medium became considerably viscous with the consumption of lactose. Paper-chromatographic analyses of the culture broth indicated that lactose was not hydrolyzed to its constituent monosaccharides during fermentation and the amount of produced polysaccharide reached 27.12% of the furnished lactose after 8 days. Without aeration, however, it was observed that the viscosity of the medium did not increase and no polysaccharide was produeed.2) Crude polysaccharide was easily separated from the broth by adding 3 vol. of ethanol and a small amount of Ba(OH)_2 solution, and then it was deproteinized by treating with dil.alkali, followed by SAVEG's procedure. After precipitation with ethanol, the polysaccharide was extracted with hot ethanol (70% v/v), dialyzed and reprecipitated by pouring into 5 vol.of ethanol. Thus a purified material ([α]^<?5>_D-62.0) which contained only 0.05% nitrogen was obtained.On the other hand, a partially depolymerized and purified polysaccharide was obtained as copper-complex after treatment with HCl (pH 2.0) at 70℃.
社団法人日本生物工学会の論文
1958-01-15

Bacillus polymyxa1変株の生成するBacterial Polysaccharideの研究(第7報) : タンク培養に依る生産(其の1)

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