Endomyces sp.によるglucamylase : 生産のスケールアップ

概要

論文の詳細を見る
Studies on the effects of aeration and agitation in glucamylase fermentation using Endomyces sp. with 30l, 100l, 5t and 50t fermenters were carried out, and the following results were obtained. (1) The agreement between the oxygen uptake and the oxygen demand was significantly better in fermentations performed at higher agitation rate. It appears reasonable to ascribe the higher yield of glucamylase to the fulfilment of the oxygen demand in the growing phase. (2) The correlation between the yield of glucamylase and the oxygen transfer rate was clear. The oxygen transfer rate was indicated by k_dP, which is the prodct of the volumetric oxygen transfer coefficient (k_d) by the total pressure in fermenter (P). The desirable yield was obtained above the critical k_dP value of 0.65 mM O_2/l/min. (3) THe correlation between k_La and process variables was checked in four scale fermenters, and the following experimental equation was obtained at the modified Cooper's equation; k_La=1.78(P_g/V)^<0.33>V<0.56>_s where k_L : over-all coefficient of mass transfer based on liquid film, a : interfacial area between bubbles and liquid per unit volume of liquid, P_g/V : power input per unit volume of gassed liquid, V_s : linear velocity of air based on the empty cross-sectional area of the vessel. It was found that the above equation could be used in scale-up of fementers. However, the correlations of Humphrey and Richard could not be applied to this system. The experimental correlation between power requirement with and without aeration consisted with Michel's equation. (4) In 50 t fermenter, the oxygen transfer coefficient increased from 1.14 to 1.58 by the reconstruction of turbine impeller from 79 cm to 94 cm diameter. The yield of enzyme production also increased by 30 %.
社団法人日本生物工学会の論文
1968-10-25

Endomyces sp.によるglucamylase : 生産のスケールアップ

スポンサーリンク

概要

著者

関連論文

スポンサーリンク