微生物の酵素生産に関する基礎的研究

概要

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A number of useful enzymes have been produced in industry using specific microorganisms. To enhance the enzyme productivity, two basic approaches are available : (1) control of environmental factors such as cultivation temperature, pH, and substrate concentration; (2) genetic engineering, i.e., cloning of the enzyme gene and the optimum control of the gene expression. Using these techniques, enzyme productivity can be enhanced. The following examples are discussed.(1) Optimization of α-galactosidase production by mold.(2) Transformation of a thermophile, Bacillus stearothermophilus, with plasmid DNA.(3) Molecular cloning of thermostable enzyme genes (α-amylase, neutral protease) in B. stearothermophilus and Bacillus subtilis.(4) Cloning of the penicillinase genes of Bacillus licheniformis in Escherichia coli, B. subtilis, B. licheniformis, and B. stearothermophilus.(5) Exploitation of protein secretion vectors using the penicillinasc gene.
社団法人日本生物工学会の論文
1988-05-25