連続醗酵によるグルタミン酸生産に関する研究 : (第4報)細胞再使用系連続醗酵の動力学的解析

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In previous report, new concept on the activity distribution function of the cell population had been introduced to the dynamic analysis of continuous L-glutamicacid fermentation with Brevibacterium sp. in order to design successfully the production stages of the multiple stage continuous fermentation and we had found that the mean fermentation rate and the mean completion of fermentation behave in opposite ways with regards to the mean residence time of the system. These results seemed to suggest us the importance of the concept of "cell activity reuse system" or "cell recycling continuous fermentation" for the purpose to increase the activity utilization of cell population.On the basis of equation (1) and (2) which defined the mean fermentation rate and mean completion of fermentation in the steaday state, the cell recycling continuous fermentation was classified into two groups. -direct recycling method without activation culture and indirect recycling one with activation culture.In the dynamic analysis of the direct recycling method, the residence time distribution functions of various recycling continuous systems were developed by Laplace inverse transformation of transfer function of each system, and equations (3)〜(16) were obtained.A new method to predict the general mean fermentation rate and the mean completion of fermentation in the steady state recycling continuous fermentation was derived from above results.Analyzing the equations (17) and (18) in the recycling system of two vesesls, for example, we found that both recycling efficiencies which were newly defined with equations (19) and (20) could be incresed, if adequate recycling ratio λ (=Ψ/(F+Ψ)) was given-Figures 4,5 and 6. The decreased L-glutamic acid production activity of cell population in the production culture could be reactivated, as wasi llustrated in Figures 7 and 8,when the cell population at the sutable caltureage was shifted up to nutrious culture (activation culture.) Some physiological experiments on the reactivation phenomena of the cell population showed that the mean cell mass and RNA/DNA were sharply increased during the activation culture. We considered that the cellular nucleic acid level or the mean cell mass would be possibly used to discuss the biotechnological problems such as scale up of fermentation process, continuous fermentation etc.The theoretical prediction equation for indirect recycling continuous fermentation may be obtaind by using probability theory.
社団法人日本生物工学会の論文
1964-11-25

連続醗酵によるグルタミン酸生産に関する研究 : (第4報)細胞再使用系連続醗酵の動力学的解析

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