Bacillus polymyxa1変株の生成するBacterial Polysaccharideの研究(第6報) : Chromatographyに依る構成糖の分離及び定量

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Methylpentose and aldohexose, which were shown in the previous study as the components of the bacterial polysaccharide, have been isolated as crystals from the acid-hydrolysate by cellulose powder column chromatography. As the results of examining the characteristics of the isolated sugars, almost all parts of methylpentose were identified as l-rhamnose, and those of aldohexose as d-galactose.On the other hand, a fucose-like methylpoentose, mannose, and hexosamine (0.02〜0.05% colorimetrically determined) were detected.Furtheremore, it has been found by colorimetric determination after paper chrometographic separation that the polysaccharide consists of 66.8〜67.9% of l-rhamnose and 30.4〜31.5% of d-galactose.The above-mentioned results indicate that this polysaccharide should be called a bacterial rhamnogalactan.
社団法人日本生物工学会の論文
1958-01-15