食品中の核酸成分に関する研究 : (第15報)シイタケの冷凍における核酸成分の消長

概要

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In the previous paper, changes of 5'-nucleotides and nucleoside polyphosphate in mushrooms (Psalliota bisporus) during their cold storage and defrosting were reported. It seems interesting to compare the change of nucleotide content in Shii-take (Lentinus edodes) with that in mushrooms (P. bisporus), because the enzymatic activities of Shii-take decomposing nucleic acid are different from those of mushrooms. The amount of 5'-nucleotides in frozen Shii-take at -20℃ was determined by ion exchange chromatography and 5'-nucleotidase assay.A remarkable decrease of ATP and a slight increase of 5'-nucleotides were found during the defrosting process of Shii-take at 5℃ for 24 hr or at 35℃ for 2 hr. Under storage at -20℃ for 6 months, the amount of ATP decreased gradually, but no increase of each 5'-nucleotide was observed. Activities of RNase adn PDase remained rather stable in frozen samples stored after 6 months' cold storage. Authentic ATP was degraded to 5'-AMP and adenosine at a low temperature in a crude enzyme solution extracted from frozen Shii-take stored for 6 months. These facts may substantiate the possibility of the formation of AMP and adenosine by a decrease of ATP during the prolonged cold storage of Shii-take.
社団法人日本生物工学会の論文
1969-02-25