Bacillus polymyxa 1変株の生成する Bacterial Polysaccharideの研究 (第10報) : Acetyl及びMethyl誘導体の調製

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1) Acertylation of the polysaccharide was accomplished with pyridine and acetic anhydride after pretreatment wit formamide, through it was confirmed by viscosity measurement that the extensive degradation had occured during acetylation with BARNETT's catalyst. The acetate is a white amorphous substance, (CH_3CO-; 42.70%) with m.p. 248℃, [α]^<20>_D-58.0 in chloroform.2) The methyl derivative was prepared from free the polysaccharide or its acetate by means of successive methylation with dimethylsulfate and sodium hudroxide. And after extracting with chloroform, it was purified by fractionation with petroleum ether.In the case of methylation of free polysaccharide, methoxyl groups were not fully incorporated (CH_<3O>- ;36.7%) by ten times repeated methylation.However, the fully methylated polysaccharide was obtained from the acetate by five times repeated treatment. The methyl ether is a pale yellow glass (CH_sO-; 38.1%) with m.p. 191〜192℃, [α]^<20>_D-76.6 in chloroform.3) The methylated polysaccharide prepared from the acetate was preliminarily methanolyzed and hydrolyzed. The following five methyl sugar components were detected by paper chromatography of the hydrolysate : tri-methyl-rhamnose, di-mthyl-rhamnose, trimethyl-galactose, mono-methyl-rhamnose and di-methyl-galactose. (Each sugar has not been isolated yet.)This fact suggests that the polysaccharide (rhamnogalactan) has a branched structure, and the non-reducing terminal groups are composed of l-rhamnose.
社団法人日本生物工学会の論文
1958-05-15

Bacillus polymyxa 1変株の生成する Bacterial Polysaccharideの研究 (第10報) : Acetyl及びMethyl誘導体の調製

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