食品中の核酸成分に関する研究 : (第3報)水産食品における5'-ヌクレオチドの分布

概要

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Distributions of 5'-nucleotides in sea foods, namely in shellfishes, squids, shrimps and crabs, were investigated in order to learn these role on flavor. Individual distribution of 5'-nucleotide in perchloric acid extracts of three kinds of Crustacea and five kinds of Mollusca were determined by ion-exchange column chromatography. Six fractions were obtained by Dowex I×8 column from the extract of raw shrimp. Among them two fractions were identified as 5'-adenylic acid (5'-AMP) and 5'-inosinic acid (5'-IMP). Also the presence of 5'-AMP and 5'-uridylic acid (5'-UMP) was recognized in boiled Asari (Venerupis semidecusata). As the results of column chromatography, 5'-AMP and 5'-UMP were found to be the major components belonging to 5'-nucleotides, of Crustacea and Mollusca. 5'-cytidylic acid was found in several cases. but only small amounts of 5'-guanylic acid were detected. 5'-IMP was found in very small quantities, except in shrimp (264 μmole/100g in shrimp extract). Samples of sea foods were mostly found to contain 15-150 μmole/100g material of a total of 5'-nucleotide. It was supposed that 5'-AMP may contribute to the flavor of Sea foods in conjunction with glutamic acid.
社団法人日本生物工学会の論文
1965-01-25