Location of Two Photoaffinity-Labeled Sites on the Ligand-Binding Domain of Retinoic Acid Receptor α

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概要

• 論文の詳細を見る

• Retinoic acid receptors (RARs) consist of six domain structures. The C-terminal region (D/E/F-domains) is involved in ligand binding, dimerization, and ligand-dependent transactivation. Structural information about RARs is required for understanding its complex function. A photoreactive retinoid denoted as ADAM-3,which was designed as the result of comparison of two fluorescent retinoids (DAM-3 and DAM-15), was synthesized and used for photoaffinity labeling of recombinant protein MBP-RARα/E. The photoaffinity-labeled site was determined by an endoprotease combination method which utilizes four endoproteinases in a two-phase digestion procedure. Two major labeled fragments were detected in each digestion, and the results of two-phase digestion allowed identification of the labeled residues as being located within residues 492-510 and 585-594,which correspond to 288-306 and 381-390 in human RARα, respectively.

• 公益社団法人日本薬学会の論文
• 1996-05-15

著者

• FUKASAWA Hiroshi

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• HASHIMOTO Yuichi

  Institute of Molecular & Cellular Biosciences, The University of Tokyo

• SHUDO Koichi

  Faculty of Pharmaceutical Sciences, The University of Tokyo

• Sasaki T

  School Of Pharmaceutical Sciences Toho University

• Shudo Koichi

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Shudo Koichi

  Faculty Of Pharmaceutical Science Kyushu University

• IWASAKI Shigeo

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• FUKASAWA Hiroshi

  Faculty of Pharmaceutical Sciences, University of Tokyo

• SASAKI Toru

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• Shudo K

  Research Foundation Itsuu Laboratory

• SHIMAZAWA Rumiko

  Institute of Molecular & Cellular Biosciences, The University of Tokyo

• Fukasawa Hiroshi

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Iwasaki S

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Iijima Toru

  Faculty Of Pharmaceutical Sciences The University Of Tokyo

• SAWADA Takayuki

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• Fukasawa H

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Iwasaki S

  Univ. Tokyo Tokyo

• Sawada Takayuki

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Sawada Takayuki

  Institute Of Molecular And Cellular Biosciences (imcb) The University Of Tokyo

• Iwasaki Shigeo

  Institute Of Molecular And Cellular Biosciences (imcb) The University Of Tokyo

• Sasaki Toru

  Institute Of Fluid Science Tohoku University

• IWASAKI Shigeo

  Institute of Molecular and Cellular Bioscience, The University of Tokyo

• SASAKI Toru

  Institute of Applied Microbiology, The University of Tokyo

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