Structure-Based Design and Synthesis of Fluorescent PPARα/δ Co-agonist and Its Application as a Probe for Fluorescent Polarization Assay of PPARδ Ligands
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概要
- 論文の詳細を見る
Based on the result of X-ray crystallographic analysis of our peroxisome proliferator-activated receptor alpha and delta(PPARα/δ)co-agonist complexed with human PPAR ligand binding domain(LBD), we designed and synthesized an optically active fluorescent PPARα/δ co-agonist, which has a pyrene unit incorporated directly at the hydrophobic tail part of the structure as a fluorophore. This fluorescent co-agonist was applied in a homogeneous fluorescent polarization assay format for the identification of PPARδ ligands.
- 公益社団法人日本薬学会の論文
- 2008-09-01
著者
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MIYACHI Hiroyuki
Institute of Molecular & Cellular Biosciences, The University of Tokyo
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HASHIMOTO Yuichi
Institute of Molecular & Cellular Biosciences, The University of Tokyo
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Hashimoto Yuichi
Univ. Tokyo Tokyo Jpn
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Kasuga Jun-ichi
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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Hashimoto Y
Univ. Tokyo Tokyo Jpn
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Hashimoto Y
Graduate School Of Pharmaceutical Sciences Kyushu University
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ARAYA Yoko
Institute of Molecular and Cellular Biosciences, University of Tokyo
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TOYOTA Kenji
The Takara-Bio Endowed Division, Institute for Protein Research, Osaka University
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HIRAKAWA Yuko
The Takara-Bio Endowed Division, Institute for Protein Research, Osaka University
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OYAMA Takuji
The Takara-Bio Endowed Division, Institute for Protein Research, Osaka University
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MAKISHIMA Makoto
School of Medicine, Nihon University
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MORIKAWA Kosuke
The Takara-Bio Endowed Division, Institute for Protein Research, Osaka University
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Miyachi Hiroyuki
Institute Of Molecular & Cellular Biosciences University Of Tokyo
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Hashimoto Yuichi
Institute Of Molecular & Cellular Biosciences University Of Tokyo
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Hashimoto Yuichi
Institute Of Molecular And Cellular Biosciences The Universityv Of Tokyo
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Araya Yoko
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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Oyama Takuji
The Takara-bio Endowed Division Institute For Protein Research Osaka University
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Toyota Kenji
The Takara-bio Endowed Division Institute For Protein Research Osaka University
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Hirakawa Yuko
The Takara-bio Endowed Division Institute For Protein Research Osaka University
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Harada Yuichi
Center For Influenza Virus Research National Institute Of Infectious Diseases
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Morikawa Kosuke
The Takara-bio Endowed Division Institute For Protein Research Osaka University
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Miyachi H
Institute Of Molecular & Cellular Biosciences The University Of Tokyo
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Miyachi Hiroyuki
Div. Of Pharmaceutical Sciences Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Ok
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Hashimoto Y
Institute Of Molecular & Cellular Biosciences The University Of Tokyo
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Makishima Makoto
Div. Of Biochemistry Dep. Of Biomedical Sciences School Of Medicine Nihon Univ.
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Hashimoto Yuichi
Institute of Molecular & Cellular Biosciences, The University of Tokyo
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