Photoaffinity Labeling of Tumor Promoter-Binding Protein (CN-TPBP) and Preparation of Affinity Sorbent Gels

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概要

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• Cytosolic-nuclear tumor promoter-specific binding protein (CN-TPBP) was photoaffinity-labeled with a specific ligand, 3β, 5α-dihydroxycholestan-6-one (YS-64). Analysis by ODS-HPLC of peptide fragments obtained from the labeled CN-TPBP by trypsinization indicated the existence of a single specifically labeled site. Affinity gels for the purification of CN-TPBP were then prepared. As ligands for the affinity gels, 12-O-tetradecanoylphorbol 13-acetate (TPA), a typical phorbol-type tumor promoter, and benzolactam-V8-310 (BL-V8-310), a structural/biological mimic of teleocidin-class tumor promoters, were adopted. The use of these gels afforded a protein that showed a single band of 58 kDa on SDS-PAGE.

• 社団法人日本薬学会の論文
• 1997-01-15

著者

• 遠藤 泰之

  東北薬科大学薬学部

• FUKASAWA Hiroshi

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• HASHIMOTO Yuichi

  Institute of Molecular & Cellular Biosciences, The University of Tokyo

• ENDO Yasuyuki

  Faculty of Pharmaceutical Sciences, The University of Tokyo

• SHUDO Koichi

  Faculty of Pharmaceutical Sciences, The University of Tokyo

• Shudo Koichi

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Shudo Koichi

  Faculty Of Pharmaceutical Science Kyushu University

• IWASAKI Shigeo

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• AZUMA Akihiko

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• TAKEHANA Shunji

  Faculty of Pharmaceutical Sciences, University of Tokyo

• ANDO Yunike

  Institute of Molecular & Cellular Biosciences, University of Tokyo

• YAMAGUCHI Mitsuhiro

  Faculty of Pharmaceutical Sciences, University of Tokyo

• FUKASAWA Hiroshi

  Faculty of Pharmaceutical Sciences, University of Tokyo

• Shudo K

  Research Foundation Itsuu Laboratory

• Fukasawa Hiroshi

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Iwasaki S

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Yamaguchi M

  Gifu Pharmaceutical University

• Ando Yunike

  Institute Of Molecular & Cellular Biosciences University Of Tokyo

• Azuma Akihiko

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Fukasawa H

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• 山口 雅久

  Fuji-gotemba Research Laboratories Chugai Pharmaceutical Company Ltd.

• Takehana Shunji

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• Iwasaki S

  Univ. Tokyo Tokyo

• Endo Yasuyuki

  Faculty Of Pharmaceutical Sciences Tohoku Pharmaceutical University

• Iwasaki Shigeo

  Institute Of Molecular And Cellular Biosciences (imcb) The University Of Tokyo

• IWASAKI Shigeo

  Institute of Molecular and Cellular Bioscience, The University of Tokyo

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