Isolation of Deoxycytidine Kinase from Ehrlich Carcinoma Cells by Affinity Chromatography Based on a Substrate Analog, 2'-C-Cyano-2'-deoxy-1-β-D-arabinofuranosyl-N^4-palmitoylcytosine

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概要

• 論文の詳細を見る

• Deoxycytidine kinase from Ehrlich carcinoma cells was purified 10400-fold by ammonium sulfate fractionation and affinity chromatography using Sepharose 4B coupled to 2'-C-cyano-2'-deoxy-1-β-D-arabinofuranosyl-N^4-palmitoylcytosine, with a yield of 45%. The purified enzyme preparation showed a single major band with a molecular weight of 32000 on SDS-PAGE. The enzyme phosphorylated deoxyadenosine, deoxyguanosine, cytidine, and several deoxycytidine analogues as well as deoxycytidine. Also, the kinetic parameters of the enzyme for the substrates were estimated.

• 社団法人日本薬学会の論文
• 1999-01-15

著者

• 遠藤 泰之

  東北薬科大学薬学部

• UCHIDA Hiroyuki

  Department of Earth and Space Science, Osaka University

• Hayashi Y

  Taiho Pharmaceutical Co. Ltd. Tokushima Jpn

• MATSUDA Akira

  Faculty of Pharmaceutical Sciences, Hokkaido University

• SASAKI Takuma

  Department of Experimental Therapeutics, Kanazawa University Cancer Research Institute

• Matsuda A

  Graduate School Of Pharmaceutical Sciences Hokkaido University

• Matsuda A

  Faculty Of Pharmaceutical Sciences Hokkaido University

• OBATA Tohru

  Department of Experimental Therapeutics, Cancer Research Institute, Kanazawa University

• ENDO Yoshio

  Department of Experimental Therapeutics, Cancer Research Institute, Kanazawa University

• CHEN Yi-Xin

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University

• MORINAGA Hidetaka

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University

• HAYASHI Yuko

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University

• Chen Y‐x

  Fukui Univ. Fukui Jpn

• Obata Tohru

  Department Of Experimental Therapeutics Cancer Research Institute Kanazawa University

• Uchida Hiroyuki

  Faculty Of Engineering Fukui University

• Uchida Hiroyuki

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui

• Obara Takumi

  Department Of Experimental Therapeutics Cancer Research Institute Kanazawa University

• Matsuda Akira

  Department Of Pharmaceutical Sciences Faculty Of Pharmaceutical Sciences

• Matsuda A

  Hokkaido Univ. Sapporo

• Matsuda Akira

  The Department Of Internal Medicine Kin-ikyo Chuo Hospital

• Uchida H

  Department Of Physics Kyushu University

• Chen Yi-xin

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University

• Obata Takaaki

  Department Of Experimental Therapeutics Cancer Research Institute Kanazawa University

• Sasaki Takuma

  Department Of Experimental Therapeutics Cancer Research Insitute Kanazawa University

• Morinaga Hidetaka

  Department Of Medicine And Bioregulatory Science (third Department Of Internal Medicine) Graduate Sc

• Morinaga Hidetaka

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University

• Endo Yoshio

  Department Of Expenmental Therapeutlcs Cancer Research Institute Kanazawa University

• Matsuda Akira

  Faculty Of Pharmaceutical Sciences Hokkaido University

• Uchida Hiroyuki

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Fukui

• Uchida Hiroyuki

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University

• Sasaki Takuma

  Department Of Expenmental Therapeutlcs Cancer Research Institute Kanazawa University

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