Purification and Some Properties of Plant Endonuclease from Scallion Bulbs
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概要
- 論文の詳細を見る
A plant endonuclease with 3'-nucleotidase activity was purified from scallion bulbs to homogeneity as judged by SDS-PAGE. The molecular weight of the enzyme was estimated to be 38,000 by SDS-PAGE and 40,000 by Sephadex G-100 gel filtration. The enzyme rapidly hydrolyzed yeast RNA and denatured calf thymus DNA to acid-soluble substances, and hydrolyzed the plasmid pBR322 to yield small DNA fragments at low enzyme concentrations. These four activities were eliminated by treatments with EDTA and tetraethylenepentamine. The enzyme had maximum activity at pH 8.5-9.0 for 3'-AMP, 3'-GMP, and 3'-UMP, at pH 6.5 for 3'-CMP and yeast RNA, and at pH 6.0 for denatured calf thymus DNA and pBR322. During digestion of yeast RNA by the enzyme at pH 6.5, 5'-GMP was released most rapidly, followed by 5'-UMP, 5'-AMP, and 5'-CMP. These properties were different from those of endonucleases isolated from other sources such as mung bean sprouts and wheat seedlings.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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UCHIDA Hiroyuki
Department of Earth and Space Science, Osaka University
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NOMURA Akihiko
Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University
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Uchida Hiroyuki
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui
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Wu Yi-dong
Department Of Applied Chemistry And Biotechnology Faculty Of Enginerring Fukui University
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Uchida H
Fukui Univ. Fukui Jpn
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Takadera Masayuki
Department of Applied Chemistry and Biotechnology, Faculty of Enginerring, Fukui University
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Miyashita Sachie
Department of Applied Chemistry and Biotechnology, Faculty of Enginerring, Fukui University
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Nomura Akihiko
Department Of Applied Chemistry And Biotechnology Faculty Of Enginerring Fukui University
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Miyashita Sachie
Department Of Applied Chemistry And Biotechnology Faculty Of Enginerring Fukui University
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Takadera Masayuki
Department Of Applied Chemistry And Biotechnology Faculty Of Enginerring Fukui University
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Uchida Hiroyuki
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Fukui
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Uchida Hiroyuki
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
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