Protease-catalyzed peptide formation under high pressure.
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概要
- 論文の詳細を見る
The effect of high pressure on peptide formation by the catalysis of carboxypeptidase Y (substitution of ester or peptide by amino acid derivative) or by thermolysin (condensation of <I>N</I>-acylamino acid and amino acid amide) was studied. The carboxypeptidase Y-catalyzed substitution reaction of <I>N</I>-[3-(2-furyl)acryloyl]phenylalanine ethyl ester with glycinamide or phenylalaninamide showed a six-fold higher total peptide yield at 200 MPa than at atmospheric pressure. In the case of the reaction of <I>N</I>-acyldipeptide and amino acid amide, both the peptide yield and substitution efficiency were improved at elevated pressure and the wasteful hydrolysis of the substrate was highly depressed by increasing pressure. The pressure was also effective to get rid of the substrate inhibition by the amino acid ester in the reaction between the <I>N</I>-acylamino acid ester and the amino acid ester and to yield much dipeptide ester at high pressure. An improvement of the peptide yield by pressure for the reaction of thermolysin was observed in a combination of less specific substrates, <I>N</I>-benzyloxycarbonyl-L-aspartic acid and phenylalanine methyl ester, since the high catalytic activity of this enzyme under elevated pressure was significant only in the case that the peptide yield was kinetic-controlled.
- 公益社団法人 日本化学会の論文
著者
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Kunugi Shigeru
Department Of Biomolecular Engineering Graduate School Of Science And Technology Kyoto Institute Of
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Nomura Akihiko
Department Of Applied Chemistry And Biotechnology Faculty Of Enginerring Fukui University
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Yamashita Kouji
Department Of Surgery Nippon Medical School Second Hospital
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Morikawa Yoshio
Department of Applied Chemistry, Fukui University
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Tanabe Kazuo
Department of Applied Chemistry, Fukui University
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Ito Takanobu
Department of Applied Chemistry, Fukui University
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Kondoh Toshihiro
Department of Applied Chemistry, Fukui University
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Hirata Kouichirou
Department of Applied Chemistry, Fukui University
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Tanabe Kazuo
Department of Applied Chemistry
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Kunugi Shigeru
Department of Applied Chemistry, Fukui University
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Kunugi Shigeru
Department of Applied Chemistry
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Nomura Akihiko
Department of Applied Chemistry, Fukui University
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