Proton inventory of carboxypeptidase Y-catalyzed reactions.
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概要
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The partial solvent isotope effect of D<SUB>2</SUB>O on the carboxypeptidase Y-catalyzed hydrolysis of a peptide (3-(2-furyl)acryloyl-L-phenylalanyl-L-phenylalanine) and an ester (3-(2-furyl)acryloyl-L-phenylalanine ethyl ester) was investigated. The peptide hydrolysis showed a smaller kinetic isotope effect regarding the catalytic rate constant (<I>k</I><SUB>cat</SUB>) and the second-order rate constant (<I>k</I><SUB>cat</SUB>⁄<I>K</I><SUB>m</SUB>). <I>k</I><SUB>cat</SUB> Varied linearly on the heavy-water content, indicating that one proton is essentially transferred during the rate-determining step. On the other hand, an ester hydrolysis showed a larger, nonlinear effect, indicating the possibility of a transfer of two or more protons in the rate-determining step of the ester hydrolysis, possibly the deacylation step.
- 公益社団法人 日本化学会の論文
著者
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Fukuda Mitsuhiro
Department Of Neurophysiology Biomedical Research Center Osaka University Medical School
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Tanabe Kazuo
Department of Applied Chemistry
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Kunugi Shigeru
Department of Applied Chemistry
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