Cloning and Expression of Three Formate Oxidase Genes from Debaryomyces vanrijiae MH201

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概要

• 論文の詳細を見る

• Debaryomyces vanrijiae MH201 produces formate oxidase (FOD) at estimated pI values by native isoelectric focusing of 5.1, 5.4, and 5.9. We cloned and expressed three formate oxidase cDNAs, FOD1, FOD2, and FDO3, of the yeast using Escherichia coli. The open reading frames of FOD1, FOD2, and FDO3 were 1,731 bp long, and encoded 576-amino acid polypeptides with molecular masses of 64,142, 63,794, and 63,836 Da respectively. Expression of FOD1, FOD2, and FOD3 resulted in the production of three isozymes, with pI values of 5.1, 5.9, and 5.9 respectively. Co-expression of FOD1 and FOD2 and of FOD1 and FOD3 resulted in the production of additional isozymes with pI values, of 5.4. The three amino acid sequences of FOD1, FOD2, and FOD3 contained a consensus motif of a flavin adenine dinucleotide binding site in their N-terminal parts and a glucose-methanol-choline oxidoreductase signature pattern, suggesting that formate oxidase ought to be classified in the glucose-methanol-choline oxidoreductase family.

• 社団法人 日本農芸化学会の論文
• 2008-08-23

著者

• FUJII Yutaka

  Department of Applied Physics, University of Fukui

• UCHIDA Hiroyuki

  Department of Earth and Space Science, Osaka University

• Fujii Yutaka

  Department Of Applied Physics University Of Fukui

• Fujii Yutaka

  Dep. Of Molecular Biology And Chemistry Fac. Of Medicine Univ. Of Fukui

• Uchida Hiroyuki

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui

• Maeda Yoshifumi

  Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk

• Oki Masaya

  Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk

• Oki Masaya

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui

• Maeda Yoshifumi

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui

• Hatanaka Akira

  Department of Applied Chemistry & Biotechnology, Graduate School of Engineering, University of Fukui

• HOJO Masayuki

  Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk

• HIRANO Kouzou

  Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk

• Hatanaka Akira

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui

• Hojo Masayuki

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui

• Hirano Kouzou

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui

• Uchida Hiroyuki

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Fukui

• Uchida Hiroyuki

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University

• Hatanaka Akira

  Department Of Applied Chemistry & Biotechnology Graduate School Of Engineering University Of Fuk

• Oki Masaya

  Department Of Applied Chemistry & Biotechnology Graduate School Of Engineering University Of Fuk

• Fujii Yutaka

  Deparment Of Bacteriology Hiroshima University School Of Medicine

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