Cloning of a Novel Dehalogenase from Envirohmenial DNA
スポンサーリンク
概要
- 論文の詳細を見る
Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.
- 社団法人 日本農芸化学会の論文
- 2010-06-23
著者
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Yamaguchi Sachiko
Daikin Industries Ltd. Environmental Technol. Lab.
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Fujii Yutaka
Department Of Applied Physics University Of Fukui
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Kimoto Hisashi
Department Of Bioscience Fukui Prefectural University
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Kimoto Hisashi
Department Of Applied Physics And Chemistry Fukui Institute Of Technology
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Taketo Akira
Department Of Applied Physice And Chemistry
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Suye Shin-ichiro
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk
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Taketo Akira
Department Of Environmental And Biotechnological Frontier Engineering Fukui University Of Technology
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Yoshioka Toshihito
Department Of Bioscience Fukui Prefectural University
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MAKISHIMA Hirokazu
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk
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ARAI Jun-ichirou
Daikin Industries, Ltd., Environmental Technology Laboratory
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Fujii Yutaka
Department Of Molecular Biology And Chemistry Faculty Of Medicine University Of Fukui
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Yamaguchi Sachiko
Daikin Industries Ltd. Environmental Technology Laboratory
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Suye Shin-ichiro
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui
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Makishima Hirokazu
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui
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Arai Jun-ichirou
Daikin Industries Ltd. Environmental Technology Laboratory
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Yoshioka Toshihito
Department Of Bioscience Faculty Of Biotechnology Fukui Prefectural University
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Fujii Yutaka
Deparment Of Bacteriology Hiroshima University School Of Medicine
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