Purification and Properties of Malic Enzyme from Pseudomonas diminuta IFO-13182
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概要
- 論文の詳細を見る
A malic enzyme from a cell-free extract of Pseudomonas diminuta IFO-13182 was purified to electrophoretic homogeneity by DEAE-Sepharose, Sephacryl, and Blue-Sepharose chromatographies. The purified enzyme required either NAD^+ or NADP^+ as a coenzyme. From the results of coenzyme specificity, the enzyme should be classified as L-malate : NAD^+ oxidoreductase (decarboxylating) [EC 1.1.1.39]. The purified enzyme was most active at pH 7.5 and 50℃ and was stable in the pH range from 7.0 to 9.0. The isoelectric point was pH 4.3. Its molecular weight was 680,000 by COSMOSIL 5-Diol high performance liquid gel filtration on chromatography and 65,000 by SDS polyacrylamide gel electrophoresis. This indicates that the enzyme consisted of 10 subunits. The malic enzyme activity with NADP^+ was about twice measured with NAD^+.
- 社団法人日本生物工学会の論文
- 1992-05-25
著者
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OKADA Yasuhiro
Departments of Pediatric Surgery and Pediatrics, Juntendo University School of Medicine
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Okada Y
Department Of Chemical Engineering Nara National College Of Technology
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Okada Yasuhiro
Department Of Gastroenterology Fukuoka Red Cross Hospital
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Suye Shin-ichiro
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk
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FUNADA ARIO
Department of Chemical Engineering, Nara National College of Technology
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KAWAGOE MIKIO
Department of Chemical Engineering, Nara National College of Technology
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INUTA SHUSEI
Department of Chemical Engineering, Nara National College of Technology
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Funada Ario
Department Of Chemical Engineering Nara National College Of Technology
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Kawagoe Mikio
Department Of Chemical Engineering Nara National College Of Technology
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Kawagoe M
Nara National Coll. Technol. Nara Jpn
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Suye Shin-ichiro
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui
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Suye Shin-ichiro
Department Of Chemical Engineering Nara National College Of Technology
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Inuta S
Department Of Chemical Engineering Nara National College Of Technology
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Inuta Shusei
Department Of Chemical Engineering Nara National College Of Technology
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OKADA YASUHIRO
Department of Chemical Engineering, Nara National College of Technology
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Okada Yasuhiro
Department of Applied Chemistry for Resources, Tokyo University of Agriculture and Technology
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