Reverse Reaction of Malic Enzyme for HCO_3^- Fixation into Pyruvic Acid to Synthesize L-Malic Acid with Enzymatic Coenzyme Regeneration
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概要
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Malic enzyme [L-malate: NAD(P)+ oxidoreductase (EC 1.1.1.39)] catalyzes the oxidative decarboxylation of L-malic acid to produce pyruvic acid using the oxidized form of NAD(P) (NAD(P)+). We used a reverse reaction of the malic enzyme of Pseudomonas diminuta IFO 13182 for HCO3− fixation into pyruvic acid to produce L-malic acid with coenzyme (NADH) generation. Glucose-6-phosphate dehydrogenase (EC1.1.1.49) of Leuconostoc mesenteroides was suitable for coenzyme regeneration. Optimum conditions for the carboxylation of pyruvic acid were examined, including pyruvic acid, NAD+, and both malic enzyme and glucose-6-phosphate dehydrogenase concentrations. Under optimal conditions, the ratio of HCO3− and pyruvic acid to malic acid was about 38% after 24 h of incubation at 30 °C, and the concentration of the accumulated L-malic acid in the reaction mixture was 38 mM. The malic enzyme reverse reaction was also carried out by the conjugated redox enzyme reaction with water-soluble polymer-bound NAD+.
- 社団法人 日本農芸化学会の論文
- 2008-05-23
著者
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Suye Shin-ichiro
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui
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Zheng Haitao
School of Material Science and Chemical Engineering, Tianjin Polytechnic University
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Ohno Yoko
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk
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Nakamori Toshihiko
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fuk
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Ohno Yoko
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui
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Zheng Haitao
School Of Material Science And Chemical Engineering Tianjin Polytechnic University
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Suye Shin-ichiro
Dep. Of Applied Chemistry And Biotechnology Graduate School Of Engineering Univ. Of Fukui
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Nakamori Toshihiko
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering University Of Fukui
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