Optimum Conditions for Electric Pulse-mediated Gene Transfer to Bacillus subtilis Cells(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
It was found that plasmid DNA (pUB 110) can be introduced into not only protoplasts but also intact cells of Bacillus subtilis by electric field pulses. The transformation of B. subtilis using protoplasts results in an efficiency of 2.5x10^4 transformants per μg of DNA, with a single pulse of 50μsec with an initial electric field strength of 7 kV/cm. Even transformation of intact B. subtilis cells results in a maximum efficiency of 1.5x10^3 transformants per μg DNA, with a single pulse of 400 μsec with an initial electric field strength of 16 kV/cm. The cell survival of protoplasts and intact cells was approximately 100% and 30%, respectively, under the conditions found to be optimal for the transformation process. Plasmid DNA isolated from pUB 110 containing transformants was indistinguishable from authentic preparations of pBU 110 on gel electrophoretic analysis.
- 社団法人日本農芸化学会の論文
- 1989-09-23
著者
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HAYASHI Yoshitaka
Department of Genetics, Division of Stress Adaptation and Protection, Research Institute of Environm
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Kimoto Hisashi
Department Of Bioscience Fukui Prefectural University
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Kimoto Hisashi
Department Of Applied Physics And Chemistry Fukui Institute Of Technology
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KUSAOKE Hideo
Department of Environmental and Biotechnological Frontier Engineering, Fukui University of Technolog
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Kusaoke Hideo
Department Of Applied Physics And Chemistry Fukui Institute Of Technology
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Kusaoke Hideo
Department Of Applied Physics And Chemistry Faculty Of Engineering Fukui University Of Technology
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Hayashi Yoshitaka
Department Of Genetics Research Institute Of Environmental Medicine Nagoya University
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KADOWAKI Yasuhiro
Department of Applied Physics and Chemistry, Fukui Institute of Technology
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Kadowaki Yasuhiro
Department Of Applied Physics And Chemistry Fukui Institute Of Technology
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Hayashi Yoshitaka
Department Of Applied Physics And Chemistry Fukui Institute Of Technology
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