Separation of Phosphatidylinositol 4-Phosphate from a Mixture with Phosphatidylserine and Phosphatidylinositol by Sep-Pak C_<18> Cartridge

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概要

• 論文の詳細を見る

• A simple and efficient method for the separation of phosphatidylinositol 4-phosphate (PI 4-P) from phosphatidylinositol (PI) and phosphatidylserine (PS) is described. A mixture of PI, PI 4-P and PS was injected onto a Sep-Pak C_<18> cartridge. PI and PS were flushed through the cartridge with solvent 1 [methanol-chloroform (3 : 1)] while PI 4-P remained in it. Then the cartridge was inverted, and PI 4-P was eluted backward with solvent 2 [chloroform-methanol-0.5 M aqueous ammonium hydroxide (9 : 7 : 2)].

• 公益社団法人日本薬学会の論文
• 2000-09-01

著者

• MORISAKI Naoko

  Institute of Molecular & Cellular Biosciences, The University of Tokyo

• HASHIMOTO Yuichi

  Institute of Molecular & Cellular Biosciences, The University of Tokyo

• Hashimoto Yuichi

  Univ. Tokyo Tokyo Jpn

• Hashimoto Y

  Univ. Tokyo Tokyo Jpn

• Morisaki N

  Institute Of Molecular & Cellular Biosciences The University Of Tokyo

• Morisaki Naoko

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Hashimoto Y

  Graduate School Of Pharmaceutical Sciences Kyushu University

• MASAOKA Miho

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• SHIRAI Ryuichi

  Research and Education Center for Materials Science, Nara Institute of Science and Technology

• Hashimoto Yuichi

  Institute Of Molecular & Cellular Biosciences University Of Tokyo

• Morisaki Naoko

  Institute Of Molecular And Cellular Bioscience The University Of Tokyo

• Masaoka Miho

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Shirai R

  Research And Education Center For Materials Science Nara Institute Of Science And Technology

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