Evaluation of Neurotoxicity of Alzheimer's Amyloid β protein (β42) in Cultured Hippocampal Cells and Its Prevention by Propentofylline
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概要
- 論文の詳細を見る
Neurotoxicity of β42(20μM) in cultured rat hippocampal neurons was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) reduction and lactate dehydrogenase(LDH) release methods as quantitative assays of cell death, and both methods indicated that propentofylline(PPF) had the ability to protect the neurons against the toxicity, although these two assay methods revealed different mechanisms for the toxic effect of β42.Promotion of the active exocytotic system of the cells was suggested after treatment with β42 in the MTT assay and in determination of 9-aminoacridine(AA) excretion from the preloaded cells after 24-h treatment with β42.The promotion of AA exocytosis was blocked by the addition of PPF(20μg/ml).The preventive effect of PPF on the neurotoxicity of β42 has been proposed to be caused by elevation of the intracellular level of cAMP as a result of depression of the hydrolytic activity of cells.
- 社団法人 日本薬理学会の論文
- 2000-04-01
著者
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Chiba Kenzo
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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Chiba Kenzo
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Koriyama Yoshiki
北陸大学 薬 生物活性
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Koriyama Yoshiki
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamazaki Matsumi
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamazaki M
School Of Pharmaceutical Sciences Kinki University
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Mohri Tetsuro
Department of Biodynamics, Faculty of Pharmaceutical Sciences, Hokuriku University
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Chiba Kenzo
Department Of Biochemistry Faculty Of Pharmaceutical Science Hokuriku University:department Of Organ
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Mohri Tetsuro
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamazaki Matsumi
Department Of Biochemistry Faculty Of Pharmaceutical Science Hokuriku University
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Kiriyama Yoshiki
Department of Biodynamics, Faculty of Pharmaceutical Sciences, Hokuriku University
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