Detection of Genipin/Geniposide-Target Molecules by a Geniposide Overlay Method Using Anti-Geniposide Antibody
スポンサーリンク
概要
- 論文の詳細を見る
A geniposide overlay method for the detection of genipin/geniposide-binding proteins was attempted using a specific anti-geniposide antiserum. The specific anti-geniposide antiserum that recognized the genipin moiety was obtained from rabbits immunized with geniposide-rabbit serum albumin (RSA) conjugate as antigen. The results of the overlay method revealed a major geniposide binding protein with a molecular weight about 170 kDa in the cytosolic fraction of rat brain cortex and PC12h cells. The major protein band was comparable with the protein band (about 170 kDa) detected by simultaneous Western blot analysis using anti-neuronal nitric oxide synthase (nNOS) antiserum. Furthermore, geniposide activated NOS activity in a concentration-dependent manner. These results indicate that the antiserum obtained was useful for the detection of a genipin/geniposide-target molecule and strongly suggested that direct binding to and activating of nNOS cause the neuritogenic activity of genipin/geniposide in PC12h cells.
- 公益社団法人日本薬学会の論文
- 2004-04-01
著者
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Chiba Kenzo
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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Chiba Kenzo
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamazaki Matsumi
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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山崎 幹夫
千葉大学薬学部
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Yoshida Akihiro
Department of Cardiovascular Medicine, Kobe University Graduate School of Medicine
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Ohkubo Tadatoshi
Kyukyu Pharmaceutical Co., Ltd.
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Mohri Tetsuro
Department of Biodynamics, Faculty of Pharmaceutical Sciences, Hokuriku University
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Ohkubo Tadatoshi
Kyukyu Pharmaceutical Co. Ltd.
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Chiba Kenzo
Department Of Biochemistry Faculty Of Pharmaceutical Science Hokuriku University:department Of Organ
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Mohri Tetsuro
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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Yoshida Akihiro
Department Of Biology Faculty Of Science Kyushu University
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Yamazaki Matsumi
Department Of Biochemistry Faculty Of Pharmaceutical Science Hokuriku University
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Yoshida Akihiro
Department Of Applied Physics Faculty Of Science Fukuoka University
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Yoshida Akihiro
Department Of Biodynamics Faculty Of Pharmaceutical Sciences Hokuriku University
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