ピロリン酸塩によるスケトウダラ・ミオシンBの解離と変性

概要

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Various concentrations of sodium pyrophosphate (0-1.0mM, PPi) together with 1.OmM MgCl2 were added to walleye pollack myosin B (MB) in 0.5M KCl containing 40mM Tris-Buffer (pH7.0). Immediately after addition of PPi-MgCl2, measurements of viscosity and ultracentrifugal sedimentation pattern indicated dissociation of a considerable amount of myosin from MB. The first order rate constants (KD) for inactivation of Ca-ATPase of the PPi-MgCl2 treated MB were estimated at2-25°C and comparison was made with those of myosin. The results showed that the inactivation rate of Ca-ATPase of the PPi-MgCl2 treated MB was largely influenced by the concentration of PPi and temperature for the treatment. As the temperature was increased along with the concentrations of PPi-MgCl2 for the treatment, the formation of myosin from MB and the rate of denaturation of Ca-ATPase also increased.
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