Sequence Analysis of Chinese and Japanese Curcuma Drugs on the 18S rRNA Gene and trnK Gene and the Application of Amplification-Refractory Mutation System Analysis for Their Authentication
スポンサーリンク
概要
- 論文の詳細を見る
The botanical origins of Chinese and Japanese Curcuma drugs were determined to be Curcuma longa, C. phaeocaulis, the Japanese population of C. zedoaria, C. kwangsiensis, C. wenyujin, and C. aromatica based on a comparison of their 18S rRNA gene and trnK gene sequences with those of six Curcuma species reported previously. Moreover, to develop a more convenient identification method, amplification-refractory mutation system (ARMS) analysis of both gene regions was performed on plants. The ARMS method for the 18S rRNA gene was established using two types of forward primers designed based on the nucleotide difference at position 234. When DNAs of four Curcuma species were used as templates, PCR amplification with either of the two primers only generated a fragment of 912 base pairs (bp). However, when DNAs of the purple-cloud type of C. kwangsiensis and C. wenyujin were used, PCR amplifications with both primers unexpectedly generated the fragment, suggesting that these two were heterozygotes. The ARMS method for the trnK gene was also established using a mixture of four types of specific reverse primers designed on the basis of base substitutions and indels among six species, and common reverse and forward primers. C. phaeocaulis or the Chinese population of C. zedoaria, the Japanese population of C. zedoaria or the purple-cloud type of C. kwangsiensis, the pubescent type of C. kwangsiensis or C. wenyujin, and C. aromatica were found to show specific fragments of 730, 185, 527 or 528, and 641 or 642 bp, respectively. All species including C. longa also showed a common fragment of 897—904 bp. Using both ARMS methods, together with information on producing areas, the identification of Curcuma plants was achieved. Moreover, the ARMS method for the trnK gene was also useful for authentication of Curcuma drugs.
- 日本薬学会の論文
- 2002-12-01
著者
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KOMATSU Katsuko
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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SASAKI Yohei
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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FUSHIMI Hirotoshi
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmace
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Cai S‐q
Department Of Natural Medicines School Of Pharmaceutical Sciences Peking University
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Cai Shao
Department Of Natural Medicines School Of Pharmaceutical Sciences Peking University
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Sasaki Y
Tohoku Pharmaceutical University
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Sasaki Y
Institute Of Natural Medicine University Of Toyama
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Cao Hui
Graduate School Of Information Science Nagoya University
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Cao Hui
Institute Of Chinese Materia Medica China Academy Of Traditional Chinese Medicine
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CAI Shao-Qing
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University
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Komatsu Katsuko
富山大学和漢医薬学総合研究所
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Komatsu K
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Komatsu K
Institute Of Natural Medical Toyama Medicine And Pharmaceutical University
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Komatsu Katsuko
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Komatsu Katsuko
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Komatsu Katsuko
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
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Komatsu K
Institute Of Natural Medicine University Of Toyama
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Fushimi Hirotoshi
Institute Of Natural Medicine University Of Toyama
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Fushimi Hirotoshi
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Fushimi Hirotoshi
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Cao Honghua
Institute Of Chinese Materia Medica China Academy Of Traditional Chinese Medicine
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Sasaki Yohei
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Sasaki Youhei
Faculty Of Pharmaceutical Sciences Hoshi University
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Sasaki Yohei
Department Of Medicinal Plant Science Hoshi University
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Cai Shao-qing
State Key Laboratory Of Natural And Biomimetic Drugs School Of Pharmaceutical Sciences Peking Univer
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Fushimi Hirotoshi
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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