Molecular Analysis of Rheum Species Used as Rhei Rhizoma Based on the Chloroplast matK Gene Sequence and Its Application for Identification (Pharmacognosy)
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概要
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Rhei Rhizoma (Dahuang in Chinese) is widely known as a purgative and antiinflammatory agent. In the Japanese Pharmacopoeia, Rhei Rhizoma is prescribed for four Rheum species, Rheum palmatum, R. tanguticum, R. officinale, and R. coreanum, while the first three species are prescribed for Dahuang in the Chinese Pharmacopoeia. Due to the morphologic similarity of the aerial parts and frequent occurrence of intermediate forms, the taxonomy of this genus and the correct identification of Rheum species and their derivative drugs are very difficult. To resolve taxonomic problems of the genus Rheum and develop an ultimate identification method for plants and drugs, molecular analysis of the chloroplast matK gene and nuclear 18S ribosomal RNA gene were performed on nine species. The sequence comparison of the matK gene revealed that most species had variable sequences not only inter- but also intraspecies. However, the specimens of the same species belonged to the same subclade in the phylogenetic tree constructed based on matK gene sequences, except for R. palmatum, in which specimens belonged to three subclades related to their production areas. The nucleotide differences at positions 587, 707, and 838 distinguished official species from others, while specific nucleotides at positions 367 and 937 became identification markers for R. palmatum, R. tanguticum, and R. officinale (or R. coreanum). Moreover, three groups of R. palmatum, each belonging to three subclades, were characterized by the nucleotides at positions 619, 769, 883, and 1061. By detecting marker nucleotides, the botanical origins of Rhei Rhizoma were determined.
- 公益社団法人日本薬学会の論文
- 2004-03-01
著者
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KOMATSU Katsuko
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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FUSHIMI Hirotoshi
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmace
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Cai S‐q
Department Of Natural Medicines School Of Pharmaceutical Sciences Peking University
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Cai Shao
Department Of Natural Medicines School Of Pharmaceutical Sciences Peking University
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CAI Shao-Qing
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University
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Komatsu Katsuko
富山大学和漢医薬学総合研究所
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Komatsu K
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Komatsu K
Institute Of Natural Medical Toyama Medicine And Pharmaceutical University
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Komatsu Katsuko
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Komatsu Katsuko
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Komatsu Katsuko
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
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YANG Dong-Ye
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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Komatsu K
Institute Of Natural Medicine University Of Toyama
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Fushimi Hirotoshi
Institute Of Natural Medicine University Of Toyama
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Fushimi Hirotoshi
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Fushimi Hirotoshi
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Yang Dong-ye
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Yang Dong
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Cai Shao-qing
State Key Laboratory Of Natural And Biomimetic Drugs School Of Pharmaceutical Sciences Peking Univer
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Fushimi Hirotoshi
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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