Rapid Detection of Panax ginseng by Loop-Mediated Isothermal Amplification and Its Application to Authentication of Ginseng(Pharmacognosy)
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概要
- 論文の詳細を見る
We have developed a novel method called loop-mediated isothermal amplification (LAMP) to detect Panax ginseng, the botanical source of Ginseng (Ginseng Radix), and to distinguish P. ginseng from Panax japonicus. Six allele-specific primers (two outer primers, two inner primers, and two loop primers) were designed based on the 18S ribosomal RNA gene sequence of P. ginseng, and LAMP was performed using those primers and total DNA extracted from P. ginseng as template. Amplifications were observed from approximately 30min onwards at DNA concentrations of 0.5 to 10.0ng. The presence of loop primers shortened the reaction time considerably. In contrast, in the reactions using total DNA from P. japonicus as template, no amplifications were observed. LAMP also enabled us to distinguish Ginseng from Japanese Ginseng (Panacis Japonici Rhizoma). LAMP was proven to be a rapid, highly sensitive, and specific method for the detection of P. ginseng and Ginseng.
- 公益社団法人日本薬学会の論文
- 2008-09-01
著者
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Sasaki Yohei
Department of Medicinal Plant Science, Hoshi University
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Nagumo Seiji
Department of Medicinal Plant Science, Hoshi University
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Komatsu Katsuko
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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Nagumo Seiji
Dep. Of Medicinal Plant Sci. Hoshi Univ.
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Komatsu Katsuko
富山大学和漢医薬学総合研究所
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine University
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Nagumo Seiji
Dep. Of Medicinal Plant Sciences Hoshi Univ.
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Sasaki Yohei
Department Of Materials Science And Technology Faculty Of Science And Technology Hirosaki University
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Sasaki Youhei
Faculty Of Pharmaceutical Sciences Hoshi University
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Sasaki Yohei
Department Of Medicinal Plant Science Hoshi University
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Nagumo Seiji
Department Of Medicinal Plant Science Hoshi University
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Komatsu Katsuko
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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