Molecular Analysis and Chemical Evaluation of Ephedra Plants in Mongolia
スポンサーリンク
概要
- 論文の詳細を見る
Ephedrae herba has been used in traditional Chinese and Japanese (Kampo) medicine from ancient times, with the primary resource being in China. In the present study, a field survey as well as molecular and chemical assessments were conducted on Ephedra plants in Mongolia to clarify whether they could be an alternative resource of the Ephedrae herba used in Japanese Kampo medicine. Ephedra sinica, E. equisetina, E. przewalskii, E. regeliana, E. monosperma and an unknown taxon (ESP) collected in Mongolia were divided into 9 genotypes on the basis of nucleotide sequences of 18S ribosomal RNA (rRNA) gene and trnK gene. E. sinica, E. equisetina, and E. monosperma presented completely identical sequences to the corresponding species from China. The sequences of trnK gene and 18S rRNA gene provide a useful index for identification and taxonomic classification of Mongolian Ephedra plants. Quantitative analysis of 5 ephedrine alkaloids revealed that almost all Mongolian Ephedra plants contained high amounts of total ephedrine alkaloids (TAs, 1.86—4.90%) and a high percentage of pseudoephedrine in TAs differed obviously from the Chinese. E. sinica and E. equisetina found in eastern and central Mongolia, showing total contents of ephedrine and pseudoephedrine higher than 1.43%, were potential new resources of Japanese Pharmacopoeia grade Ephedrae herba.
著者
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Batkhuu Javzan
Institute of Biology, National University of Mongolia
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Sanchir Chinbat
Institute of Botany, Mongolian Academy of Sciences
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Sanchir Chinbat
Institute Of Botany Mongolian Academy Of Sciences
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Shu Zhu
富山大学和漢医薬学総合研究所生薬資源科学分野
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Zhu Shu
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine University
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Kitani Yuki
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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Omote Takayuki
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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Tanaka Ken
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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Fushimi Hirotoshi
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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Mikage Masayuki
Graduate School of Natural Science and Technology, Kanazawa University
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Komatsu Katsuko
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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Zhu Shu
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
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Zhu Shu
Division Of Pharmacognosy Department Of Medicinal Resources Istitute Of Natural Medicine University
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Mikage Masayuki
Graduate School Of Natural Science And Technology Kanazawa University
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Tanaka Ken
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine University
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Kitani Yuki
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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Fushimi Hirotoshi
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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Zhu Shu
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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Komatsu Katsuko
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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Sanchir Chinbat
Institute of Biology, Mongolian Academy of Science
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Tanaka Ken
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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