Molecular Identification of "Chuanxiong" by Nucleotide Sequence and Multiplex Single Base Extension Analysis on Chloroplast trnK Gene(Pharmacognosy)
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概要
- 論文の詳細を見る
Chloroplast trnK gene sequences of Cnidium officinale and Ligusticum chuanxiong were determined to establish an effective method for identifying Japanese Senkyu and Chinese Chuanxiong, the two which have the same drug name in Chinese characters, similar external feature, but different botanical origins. Three sites of nucleotide differences were found between these 2 species at positions 767, 924 and 964 from upstream in trnK gene sequence, allowing molecular identification of the two plants and crude drugs. Further, three kinds of specific primers of 14 mer, 23 mer and 30 mer long were designed to detect these 3 sites of marker nucleotides. By using multiplex single base extension (MSBE) analysis with the 3 specific primers, C. officinale and L. chuanxio could be distinguished clearly by the electrophoretograms, where 3 peaks with different color of ddTMP, ddCMP and ddTMP were observed in case of C. officinale and those of ddGMP, ddAMP and ddGMP in L. chuanxiong. Moreover, trnK gene sequence of "Dongxiong," a kind of Chuanxiong cultivated in Northeast China, suggested that its botanical origin was C. officinale.
- 社団法人日本薬学会の論文
- 2007-03-01
著者
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Shu Zhu
富山大学和漢医薬学総合研究所生薬資源科学分野
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Zhu Shu
中華人民共和国
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Zhu Shu
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine University
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Fushimi Hirotoshi
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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Komatsu Katsuko
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
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HAN Guiru
Hebei Institute of Drugs Control
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TSUCHIDA Takashi
Kampo Research Laboratories, Kanebo Seiyaku, Ltd.
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UNO Toshio
Kampo Research Laboratories, Kanebo Seiyaku, Ltd.
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TAKANO Akihito
Showa Pharmaceutical University
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Zhu Shu
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
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Uno Toshio
Kampo Research Laboratories Kanebo Seiyaku Ltd.
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Zhu Shu
Division Of Pharmacognosy Department Of Medicinal Resources Istitute Of Natural Medicine University
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Komatsu Katsuko
富山大学和漢医薬学総合研究所
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Komatsu K
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Komatsu K
Institute Of Natural Medical Toyama Medicine And Pharmaceutical University
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Komatsu Katsuko
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Komatsu Katsuko
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
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Komatsu K
Institute Of Natural Medicine University Of Toyama
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Komatsu Katsuko
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Fushimi Hirotoshi
Institute Of Natural Medicine University Of Toyama
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Fushimi Hirotoshi
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Fushimi Hirotoshi
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Istitute Of Natural Medicine University
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Tsuchida Takashi
Kampo Research Laboratories Kanebo Seiyaku Ltd.
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Takano Akihito
Showa College Of Pharmaceutical Sciences
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Zhu Shu
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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Komatsu Katsuko
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
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