18S RIBOSOMAL RNA GENE SEQUENCES OF THREE PANAX SPECIES AND THE CORRESPONDING GINSENG DRUGS
スポンサーリンク
概要
- 論文の詳細を見る
Total DNA was extracted from the fresh underground parts of three Panax separate species. The 18SrRNA regions of extracted DNA were amplified by the polymerase chain reaction (PCR) and their sequences were determined. In each species, the sequences were found to be of 1809 bease pairs (bps) but with different gene sequences. Different base substitutions were observed at nucleotide positions 497,499,501 and 712. The same procedure was performed on commercial samples of Ginseng Radix, Panacis Japonici Rhizoma and American Ginseng. Each sequence completely corresponded with that of each original plant, namely P. ginseng, P. japonicus and P. quinquefolius, respectively. This is the first time that 18S rRNA gene sequencing on Panax species was carried out. Prevously, Ginseng drugs have been identified mainly by their external and internal structure. Thus this method will be useful in identifying Ginseng drugs at the gene level.
- 社団法人日本薬学会の論文
- 1996-11-15
著者
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磯部 正治
富山大 工 生命工学
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磯部 正治
富山医科薬科大学和漢薬研究所病態生化学
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Isobe Masaharu
富山大学 工学部
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Isobe Masaharu
Laboratory Of Molecular And Cellular Biology Department Of Materials And Biosystem Engineering Facul
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Isobe M
Molecular And Cellular Biology Laboratory Department Of Materials And Biosystem Engineering Faculty
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Isobe Masaharu
Faculty Of Engineering Toyama University
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Namba Tsuneo
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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KOMATSU Katsuko
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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FUSHIMI Hirotoshi
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmace
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Isobe Masaharu
Molecular And Cellular Biology Laboratory Department Of Materials And Biosystem Engineering Faculty
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Komatsu K
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Komatsu Katsuko
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Komatsu Katsuko
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Komatsu K
Institute Of Natural Medicine University Of Toyama
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Fushimi Hirotoshi
Institute Of Natural Medicine University Of Toyama
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Fushimi Hirotoshi
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Fushimi Hirotoshi
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Istitute Of Natural Medicine University
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Isobe M
Faculty Of Engineering Toyama University
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Isobe Masaharu
Molecular And Cellular Biology Laboratory Faculty Of Science And Engineering Graduate School Univers
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Namba T
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Namba Tsuneo
Research Institute For Wakan-yaku (traditional Sino-japanese Medicine) Toyama Medical And Pharmaceut
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Isobe Masaharu
Laboratory Of Molecular And Cellular Biology Department Of Materials And Biosystem Engineering Facul
-
Namba Tsuneo
Research Institute For Wakan-yaku (oriental Medicine) Toyama Medical And Pharmaceutical University
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磯部 正治
Molecular And Cellular Biology Laboratory Faculty Of Science And Engineering Graduate School University Of Toyama
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Fushimi Hirotoshi
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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