Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
スポンサーリンク
概要
- 論文の詳細を見る
Stemona sessilifolia, S. japonica and S. tuberosa are the three genuine sources of Stemonae Radix specified in the Chinese Pharmacopoeia (CP) for antitussive and insecticidal remedy. Significant variations in alkaloids composition and content, as well as different degree of antitussive activity were found among them. In order to accurately identify the genuine sources of Stemonae Radix in the genetic level, two polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were developed based on the sequence differences in chloroplast DNA trnL-trnF and petB-petD regions of the species recorded in CP, as well as S. parviflora and a counterfeit of Stemonae Radix, Asparagus cochinchinensis. By using the restriction enzymes MwoI, AciI and XmnI which were able to recognize specific sequence sites in the trnL-trnF region, and BclI, HincII and BslI which can recognize those in the petB-petD region to digest the corresponding PCR products, the specific digestion pattern enabled the discrimination of the botanical sources of Stemonae Radix effectively and efficiently.
- 日本薬学会の論文
著者
-
Zhu Shu
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine University
-
Komatsu Katsuko
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Univers
-
Fan Lan-Lan
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking Univ
-
Chen Hu-Biao
School of Chinese Medicine, Hong Kong Baptist University
-
Yang Dong-Hui
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking Univ
-
Cai Shao-Qing
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking Univ
-
Zhu Shu
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
-
Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
-
Cai Shao-qing
State Key Laboratory Of Natural And Biomimetic Drugs School Of Pharmaceutical Sciences Peking Univer
-
Yang Dong-hui
State Key Laboratory Of Natural And Biomimetic Drugs School Of Pharmaceutical Sciences Peking Univer
-
Chen Hu-biao
School Of Chinese Medicine Hong Kong Baptist University
-
Zhu Shu
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
-
Komatsu Katsuko
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
関連論文
- Authentication of Coscinium fenestratum among the Other Menispermaceae Plants Prescribed in Thai Folk Medicines
- Molecular Analysis of Stemona Plants in China Based on Sequences of Four Chloroplast DNA Regions
- P-41 trnK遺伝子の塩基配列に基づく刺五加の同定(2)(生薬資源・天然物化学,一般演題ポスター,第24回和漢医薬学会大会 和漢薬の複雑さ-経験知と科学知-)
- Molecular Analysis and Chemical Evaluation of Ephedra Plants in Mongolia
- Survey of Glycyrrhizae Radix resources in Mongolia : chemical assessment of the underground part of Glycyrrhiza uralensis and comparison with Chinese Glycyrrhizea Radix
- Molecular Identification of "Chuanxiong" by Nucleotide Sequence and Multiplex Single Base Extension Analysis on Chloroplast trnK Gene(Pharmacognosy)
- Simultaneous Determination of Triterpene Saponins in Ginseng Drugs by High-Performance Liquid Chromatography
- Studies of Ephedra Plants in Asia. Part 3 : The Weed Control Problem in Ephedra Cultivated Field in China
- Flavonoid Glycosides from Chromolaena odorata Leaves and Their in Vitro Cytotoxic Activity
- Application of PCR-RFLP and MASA Analyses on 18S Ribosomal RNA Gene Sequence for the Identification of Three Ginseng Drugs
- 18S RIBOSOMAL RNA GENE SEQUENCES OF THREE PANAX SPECIES AND THE CORRESPONDING GINSENG DRUGS
- Prediction of Cyclooxygenase Inhibitory Activity of Curcuma Rhizome from Chromatograms by Multivariate Analysis
- Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase
- Constituents of the seeds of Glycyrrhiza uralensis
- Sequence Analysis of Chinese and Japanese Curcuma Drugs on the 18S rRNA Gene and trnK Gene and the Application of Amplification-Refractory Mutation System Analysis for Their Authentication
- Molecular Analysis of Medicinally-Used Chinese and Japanese Curcuma Based on 18S rRNA Gene and trnK Gene Sequences
- Pharmacognostical Studies of the Chinese Folk Medicines, "Zhang ya cai(〔ショウ〕牙菜)" and "Qing ye dan(青葉胆)"
- Pharmacognostical Studies on the Traditional Drugs Used by Chinese Minority Race Called "Yi" (III) : On "Xuory" Derived from Genus Hemerocallis Plants (2)
- Pharmacognostical Studies on the Chinese Crude Drug"Fu pen zi"(覆盆子)-6-On Botanical Origin of"Fu pen zi"from Korea-3-
- Pharmacognostical Studies on the Chinese Crude Drug "Fu pen zi"-5-Botanical Origin of "Fu pen zi"from China Mainland
- Inhibitory Effects of Eleutherococcus senticosus Extracts on Amyloid β(25-35)-Induced Neuritic Atrophy and Synaptic Loss
- Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
- New Triterpenoid Saponins from the Roots of Sinocrassula asclepiadea
- Pharmacognostical Study on the Chinese"Qiang-huo(羌活)"On the Anatomical Characteristics of the Underground Parts of Notopterygium incisum and N.forbesii
- Development of a High Performance Liquid Chromatographic Method for Systematic Quantitative Analysis of Chemical Constituents in Rhubarb
- Systematic pharmacognostical study on Panax drugs and Curcuma drugs : Phylogenetic analysis, molecular authentication and quality evaluation
- Authentication of Rhei Rhizoma(Material study)
- Rapid Detection of Panax ginseng by Loop-Mediated Isothermal Amplification and Its Application to Authentication of Ginseng(Pharmacognosy)
- Genetic Profiling of Sasa Species by Analysis of Chloroplast Intron between rbcL and ORF106 and Partial ORF106 Regions(Pharmacognosy)
- Morphological, genetic, and chemical polymorphism of Curcuma kwangsiensis
- Comparative Study of Chemical Constituents of Rhubarb from Different Origins
- Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Amplification Refractory Mutation System (ARMS) Analyses of Medicinally Used Rheum Species and Their Application for Identification of Rhei Rhizoma (Pharmacognosy)
- Molecular Analysis of Rheum Species Used as Rhei Rhizoma Based on the Chloroplast matK Gene Sequence and Its Application for Identification (Pharmacognosy)
- Application of Single-Nucleotide Polymorphism Analysis of the trnK Gene to the Identification of Curcuma Plants
- Scientific basis for the anti-dementia drugs of constituents from Ashwagandha (Withania somnifera)(Chemical & Pharmacological study)
- Withanolide Derivatives from the Roots of Withania somnifera and Their Neurite Outgrowth Activities
- Protective Effect of Salvia miltiorrhiza on Angiotensin II-Induced Hypertrophic Responses in Neonatal Rat Cardiac Cells
- Trigonelline-Induced Neurite Outgrowth in Human Neuroblastoma SK-N-SH Cells
- Studies of Ephedra Plants in Asia. Part 1. On the Resources of Ephedra Plants in China
- Comparative study on volatile components of Nardostachys Rhizome
- Active components from Siberian ginseng (Eleutherococcus senticosus) for protection of amyloid β(25-35)-induced neuritic atrophy in cultured rat cortical neurons
- Genetic Diversity of Ephedra Plants in Mongolia Inferred from Internal Transcribed Spacer Sequence of Nuclear Ribosomal DNA
- Studies on morphology and aristolochic acid analogue constituents of Asarum campaniflorum and a comparison with two official species of Asari Radix et Rhizoma
- PCR-RFLP法によるビャクジュツのソウジュツに対する純度試験の妥当性確認試験
- Constituents of the seeds of Glycyrrhiza uralensis
- Pharmacognostical Studies of the Chinese Folk Medicines, "Zhang ya cai(〔ショウ〕牙菜)" and "Qing ye dan(青葉胆)"
- Pharmacognostical Study on the Chinese"Qiang-huo(羌活)"On the Anatomical Characteristics of the Underground Parts of Notopterygium incisum and N.forbesii
- Studies of Ephedra Plants in Asia. Part 3 : The Weed Control Problem in Ephedra Cultivated Field in China
- Studies of Ephedra Plants in Asia. Part 1. : On the Resources of Ephedra Plants in China
- Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography
- Active components from Siberian ginseng (Eleutherococcus senticosus) for protection of amyloid β(25-35)-induced neuritic atrophy in cultured rat cortical neurons
- Pharmacognostical Studies on the Folk Medicine in Sichuan Province in China-4-On"Ye-cai-zi(野菜子)"
- Pharmacognostical Studies on the Polygonatum Plants-2-On the Botanical Origins of the Crude Drug"Whangjeong(黄精)"from Korea
- Pharmacognostical Studies on the Chinese Crude Drug"Fu pen zi"(覆盆子)-6-On Botanical Origin of"Fu pen zi"from Korea-3-
- Pharmacognostical Studies on the Traditional Drugs Used by Chinese Minority Race Called "Yi" (III) : On "Xuory" Derived from Genus Hemerocallis Plants (2)
- Pharmacognostical Studies on the Chinese Crude Drug "Fu pen zi" (5) : Botanical Origin of "Fu pen zi" from China Mainland
- Exploration of Ephedra resource in Mongolia : From field investigation to molecular identification and chemical evaluation (Symposium in the 28th Annual Meeting of Medical and Pharmaceutical Society for WAKAN-YAKU) -- (The forefront of research on natural
- Alkylphenols from the Roots of Ardisia brevicaulis Induce G1 Arrest and Apoptosis through Endoplasmic Reticulum Stress Pathway in Human Non-small-cell Lung Cancer Cells
- Study of the Relationship between Genetics and Geography in Determining the Quality of Astragali Radix
- Systematic pharmacognostical study on Panax drugs and Curcuma drugs : Phylogenetic analysis, molecular authentication and quality evaluation
- Comparative study of the contents of analogues of aristolochic acid in two kinds of Aristolochiae Fructus by high-performance liquid chromatography
- The antidepressant effect of secoisolariciresinol, a lignan-type phytoestrogen constituent of flaxseed, on ovariectomized mice