Authentication of Rhei Rhizoma(Material study)
スポンサーリンク
概要
- 論文の詳細を見る
rights: 本文データは和漢医薬学会の許諾に基づきCiNiiから複製したものであるRhei Rhizoma (Dahuang in Chinese) is widely known as a purgative and antiinflammatory agent. In the Japanese Pharmacopoeia, Rhei Rhizoma is prescribed for 4 Rheum species, Rheum palmatum, R. tanguticum, R. officinale, and R. coreanum, while the first 3 species are prescribed for Dahuang in the Chinese Pharmacopoeia. Due to the morphologic similarity of the aerial parts, the taxonomy of this genus and the correct identification of Rheum species and their derivative drugs are very difficult. To resolve taxonomic problems of the genus Rheum and develop an ultimate identification method for plants and drugs, molecular analysis of the chloroplast matK gene and nuclear 18S ribosomal RNA gene were performed on 9 species. The sequence comparison of the matK gene revealed that most species had variable sequences not only inter- but also intraspecies. However, the specimens of the same species belonged to the same subclade in the phylogenetic tree constructed based on matK gene sequences, except for R. palmatum, in which specimens belonged to 3 subclades related to their production areas. The nucleotide differences at positions 587,707, and 838 distinguished official species from others, while specific nucleotides at positions 367 and 937 became identification markers for R. palmatum, R. tanguticum, and R. officinale. Moreover, three groups of R. palmatum, each belonging to 3 subclades, were characterized by the nucleotides at positions 619,769,883, and 1061. On the basis of the above marker nucleotides, a convenient and efficient identification method employing Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Amplification Refractory Mutation System analyses was further developed. The procedure enabled us to identify the botanic origins of 22 drug samples of Rhei Rhizoma. The matK gene sequence was valuable in identifying Rheum species and Rhei Rhizoma and in predicting their production areas, and could be used as an index for quality evaluation of Rhei Rhizoma.
- 和漢医薬学会の論文
著者
-
Cai S‐q
Department Of Natural Medicines School Of Pharmaceutical Sciences Peking University
-
Cai Shao
Department Of Natural Medicines School Of Pharmaceutical Sciences Peking University
-
Yang Dong
Division of Maternal-Feta Medicine, Department of Obstetrics and Gynecology, College of Medicine Cat
-
Komatsu K
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
-
Komatsu K
Institute Of Natural Medical Toyama Medicine And Pharmaceutical University
-
Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
-
Komatsu Katsuko
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
-
Komatsu Katsuko
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
-
KOMATSU Katsuko
Century COE Program, Toyama Medical and Pharmaceutical University
-
FUSHIMI Hirotoshi
Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University
-
Komatsu K
Institute Of Natural Medicine University Of Toyama
-
Fushimi Hirotoshi
Institute Of Natural Medicine University Of Toyama
-
Fushimi Hirotoshi
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
-
Fushimi Hirotoshi
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
-
Fushimi Hirotoshi
Department Of Kampo Pharmaceutical Sciences Nihon Pharmaceutical University
-
Yang Dong-ye
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
-
Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Istitute Of Natural Medicine University
-
Yang Dong
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
-
Yang Dong
Division Of Maternal-feta Medicine Department Of Obstetrics And Gynecology College Of Medicine Catho
-
Cai Shao-qing
State Key Laboratory Of Natural And Biomimetic Drugs School Of Pharmaceutical Sciences Peking Univer
-
Yang Dong-Ye
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
-
Komatsu Katsuko
Division of Parmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama
関連論文
- Authentication of Coscinium fenestratum among the Other Menispermaceae Plants Prescribed in Thai Folk Medicines
- Molecular Analysis of Stemona Plants in China Based on Sequences of Four Chloroplast DNA Regions
- Molecular Analysis and Chemical Evaluation of Ephedra Plants in Mongolia
- Survey of Glycyrrhizae Radix resources in Mongolia : chemical assessment of the underground part of Glycyrrhiza uralensis and comparison with Chinese Glycyrrhizea Radix
- Molecular Identification of "Chuanxiong" by Nucleotide Sequence and Multiplex Single Base Extension Analysis on Chloroplast trnK Gene(Pharmacognosy)
- Simultaneous Determination of Triterpene Saponins in Ginseng Drugs by High-Performance Liquid Chromatography
- Flavonoid Glycosides from Chromolaena odorata Leaves and Their in Vitro Cytotoxic Activity
- Application of PCR-RFLP and MASA Analyses on 18S Ribosomal RNA Gene Sequence for the Identification of Three Ginseng Drugs
- 18S RIBOSOMAL RNA GENE SEQUENCES OF THREE PANAX SPECIES AND THE CORRESPONDING GINSENG DRUGS
- Prediction of Cyclooxygenase Inhibitory Activity of Curcuma Rhizome from Chromatograms by Multivariate Analysis
- Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase
- Constituents of the seeds of Glycyrrhiza uralensis
- Fern Constituents : Triterpenoids from Adiantum capillus-veneris
- P-IS-88 Identification of Differential Gene Expression by Annealing Control Primer System and Early Up-regulations of Tissue Transglutaminase 2 and Cyclo-oxygenase I in Human Fetal Membranes of Preterm Delivery Caused by Preterm Labor(Perinatology 5,Group
- Sequence Analysis of Chinese and Japanese Curcuma Drugs on the 18S rRNA Gene and trnK Gene and the Application of Amplification-Refractory Mutation System Analysis for Their Authentication
- Molecular Analysis of Medicinally-Used Chinese and Japanese Curcuma Based on 18S rRNA Gene and trnK Gene Sequences
- Pharmacognostical Studies of the Chinese Folk Medicines, "Zhang ya cai(〔ショウ〕牙菜)" and "Qing ye dan(青葉胆)"
- Pharmacognostical Studies on the Traditional Drugs Used by Chinese Minority Race Called "Yi" (III) : On "Xuory" Derived from Genus Hemerocallis Plants (2)
- Pharmacognostical Studies on the Chinese Crude Drug"Fu pen zi"(覆盆子)-6-On Botanical Origin of"Fu pen zi"from Korea-3-
- Pharmacognostical Studies on the Chinese Crude Drug "Fu pen zi"-5-Botanical Origin of "Fu pen zi"from China Mainland
- Inhibitory Effects of Eleutherococcus senticosus Extracts on Amyloid β(25-35)-Induced Neuritic Atrophy and Synaptic Loss
- Underlying Cardiomyopathy in Patients With ST-Segment Elevation in the Right Precordial Leads(Clinical Investigation)
- Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
- New Triterpenoid Saponins from the Roots of Sinocrassula asclepiadea
- Pharmacognostical Study on the Chinese"Qiang-huo(羌活)"On the Anatomical Characteristics of the Underground Parts of Notopterygium incisum and N.forbesii
- Development of a High Performance Liquid Chromatographic Method for Systematic Quantitative Analysis of Chemical Constituents in Rhubarb
- Authentication of Rhei Rhizoma(Material study)
- Rapid Detection of Panax ginseng by Loop-Mediated Isothermal Amplification and Its Application to Authentication of Ginseng(Pharmacognosy)
- Genetic Profiling of Sasa Species by Analysis of Chloroplast Intron between rbcL and ORF106 and Partial ORF106 Regions(Pharmacognosy)
- Morphological, genetic, and chemical polymorphism of Curcuma kwangsiensis
- Comparative Study of Chemical Constituents of Rhubarb from Different Origins
- Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Amplification Refractory Mutation System (ARMS) Analyses of Medicinally Used Rheum Species and Their Application for Identification of Rhei Rhizoma (Pharmacognosy)
- Molecular Analysis of Rheum Species Used as Rhei Rhizoma Based on the Chloroplast matK Gene Sequence and Its Application for Identification (Pharmacognosy)
- Application of Single-Nucleotide Polymorphism Analysis of the trnK Gene to the Identification of Curcuma Plants
- Scientific basis for the anti-dementia drugs of constituents from Ashwagandha (Withania somnifera)(Chemical & Pharmacological study)
- Withanolide Derivatives from the Roots of Withania somnifera and Their Neurite Outgrowth Activities
- Protective Effect of Salvia miltiorrhiza on Angiotensin II-Induced Hypertrophic Responses in Neonatal Rat Cardiac Cells
- Trigonelline-Induced Neurite Outgrowth in Human Neuroblastoma SK-N-SH Cells
- Anti-invasive and anti-angiogenic activities of Curcuma sp. extracts
- Pharmacological Properties of Traditional Medivines. XXV. Effects of Ephedrine, Amygdalin, Glycyrrhizin, Gypsum and Their Combinations on Body Temperature and Body Fluid
- Pharmacological Properties of Traditional Medicines. XXIV. Classification of Antiasthmatics Based on Constitutional Predispositions
- Fern Constituents : Adiantum cuneatum. III. Four New Triterpenoids, 4,23-Bisnor-3,4-secofilic-5(24)-en-3-al, 4,23-Bisnor-3,3-dimethoxy-3,4-secofilic-5(24)-ene, 7β, 25-Epoxyfern-9(11)-en-8α-ol and 7α, 8α-Epoxyfernan-25-ol
- FERN CONSTITUENTS : TWO NEW SECOFILICANE TRITERPENOIDS FROM ADIANTUM CUNEATUM
- Comparative study on volatile components of Nardostachys Rhizome
- Genetic Diversity of Ephedra Plants in Mongolia Inferred from Internal Transcribed Spacer Sequence of Nuclear Ribosomal DNA
- Constituents of the seeds of Glycyrrhiza uralensis
- Pharmacognostical Studies of the Chinese Folk Medicines, "Zhang ya cai(〔ショウ〕牙菜)" and "Qing ye dan(青葉胆)"
- Pharmacognostical Study on the Chinese"Qiang-huo(羌活)"On the Anatomical Characteristics of the Underground Parts of Notopterygium incisum and N.forbesii
- Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography
- Active components from Siberian ginseng (Eleutherococcus senticosus) for protection of amyloid β(25-35)-induced neuritic atrophy in cultured rat cortical neurons
- Pharmacognostical Studies on the Folk Medicine in Sichuan Province in China-4-On"Ye-cai-zi(野菜子)"
- Pharmacognostical Studies on the Polygonatum Plants-2-On the Botanical Origins of the Crude Drug"Whangjeong(黄精)"from Korea
- Pharmacognostical Studies on the Chinese Crude Drug"Fu pen zi"(覆盆子)-6-On Botanical Origin of"Fu pen zi"from Korea-3-
- Pharmacognostical Studies on Adiantum Plants (IV) : On Histotaxonomy of Series Veneri-Capilliformia and the Origins of Related Crude Drugs
- Pharmacognostical Studies on the Traditional Drugs Used by Chinese Minority Race Called "Yi" (III) : On "Xuory" Derived from Genus Hemerocallis Plants (2)
- Pharmacognostical Studies on the Chinese Crude Drug "Fu pen zi" (5) : Botanical Origin of "Fu pen zi" from China Mainland
- Exploration of Ephedra resource in Mongolia : From field investigation to molecular identification and chemical evaluation (Symposium in the 28th Annual Meeting of Medical and Pharmaceutical Society for WAKAN-YAKU) -- (The forefront of research on natural