Simultaneous Determination of Triterpene Saponins in Ginseng Drugs by High-Performance Liquid Chromatography
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概要
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A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mM K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signal/noise≥3) were 0.1 μg for ginsenosides Rb_1, Re, Rd, Re and Rg_1, chikusetsusaponin III, and notoginsenoside R_2, 0.2 μg for gisenoside Ro and chikusetsusaponin IVa, 0.3 μg for chikusetsusaponin IV, and 3 μg for majonoside R_2. The calibration curve of each saponin had a correlation coefficient close to 1. Intra- and interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved.
- 社団法人日本薬学会の論文
- 2004-08-01
著者
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Shu Zhu
富山大学和漢医薬学総合研究所生薬資源科学分野
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Zhu Shu
中華人民共和国
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Zhu Shu
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine University
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ZOU Kun
Hubei Key Laboratory of Natural Products Research and Development, Three Gorges University
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ZHU Shu
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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ZOU Kun
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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CAI Shaoqing
School of Pharmaceutical Sciences, Peking University
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MESELHY Meselhy
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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KOMATSU Katsuko
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
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Cai Shaoqing
School Of Pharmaceutical Sciences Peking Univ.
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Cai Shaoqing
School Of Pharmaceutical Sciences Peking University
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Zhu Shu
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
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Zhu Shu
Division Of Pharmacognosy Department Of Medicinal Resources Istitute Of Natural Medicine University
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Komatsu K
Div. Of Pharmacognosy Dep. Of Medicinal Resources Inst. Of Natural Medicine Univ. Of Toyama
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Komatsu K
Institute Of Natural Medical Toyama Medicine And Pharmaceutical University
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Komatsu K
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine University
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Komatsu Katsuko
Division Of Pharmacognosy Department Of Medicinal Resources Institute Of Natural Medicine Toyama Med
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Komatsu Katsuko
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Komatsu Katsuko
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Komatsu Katsuko
Division Of Pharmacognosy Institute Of Natural Medicine University Of Toyama
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Meselhy Meselhy
Department Of Pharmacognosy And Medicinal Plants Faculty Of Pharmacy Cairo University
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Meselhy Meselhy
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Komatsu K
Institute Of Natural Medicine University Of Toyama
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Zhu Shu
Research Center For Ethnomedicines Institute Of Natural Medicine Research Center For Ethnomedicines
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Zou Kun
Research Center For Ethnomedicines Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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