PCR-RFLP法によるビャクジュツのソウジュツに対する純度試験の妥当性確認試験

概要

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An inter-laboratory validation study was performed for the purity test of Atractylodes Rhizome targeted for Atractylodes Lancea Rhizome based on a PCR-RFLP by 7 persons whose experience with PCR experiments ranges from 0 to 10 years. Twenty-five crude drugs derived from medicinal Atractylodes plants were distributed to each practitioner and the discrimination of them into Atractylodes Rhizome and Atractylodes Lancea Rhizome was carried out following the common experimental protocol. As a result, all practitioners achieved exact identification regardless of their experience with PCR or the kind of the instruments used in the test. This result indicates that the test is reliable for the discrimination of Atractylodes Rhizome and Atractylodes Lancea Rhizome.
2010-08-20