食品中のタール色素分析法の改良とマーケットバスケット試料への適用

概要

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In order to study the daily intake of tar dyes in children aged 1 - 6 years old using a market basket method, conventional analytical methods for 12 kinds of tar dyes in processed foods were modified and improved. In the conventional analytical methods, tar dyes are analyzed using HPLC after extraction by enzymatic treatment and purification with a solid-phase extraction cartridge. In our improved method, the preparation of sample solutions and HPLC conditions were simplified using TBA-Br solution as an ion pair reagent and the purification procedure was simplified using Sep-pak plus tC18 Environmental cartridge as a solid-phase extraction cartridge. When the improved method was applied to the analyses of tar dyes in market basket samples (Groups 1〜8), 9 colors in Group 7, i.e., Amaranth, Allura Red AC, Ponceau 4R, Acid Red, Tartrazine, Sunset Yellow FCF, Fast Green FCF, Brilliant Blue FCF and Indigotine, could not be quantified owing to interference peaks. The recoveries of the above mentioned 9 kinds of tar dyes in Group 7 were improved by adding polyamide column purification after the extraction by enzymatic treatment step. These improved methods resulted in good recoveries (72〜114%) for tar dyes, with the exception of Indigotine (24〜72%), which is unstable in processed food. The improved methods can be applied to market basket samples in the analyses of tar dyes for estimation of their daily intake amounts.
2012-08-24