大豆穀粒及び脱脂大豆の遺伝子組換えダイズ定量検知法における定量PCR法とELISA法の比較検討

概要

論文の詳細を見る
Real-time PCR method and ELISA method for quantitative determination of genetically modified soybean (CMS) and de-fatted soybean were compared. CMS contents in mixed soybean grain samples, IRMM standard, ELISA standard and IP handling soybean were determined using real-time PCR method and ELISA method. In soybean grain samples, a high correlation between GMS contents determined using real-time PCR method and ELISA method was observed. On the other hand, no correlation was observed between the GMS contents (%) determined using real-time PCR method and ELISA method in de-fatted soybean samples. These results suggested that both real-time PCR method and ELISA method would be applicable for the determination of GMS contents in soybean grain samples, but the values determined using real-time PCR method seem to be higher than those using ELISA method in de-fatted soybean samples.
日本食品化学学会の論文
2003-08-29