遺伝子組換えトウモロコシ(GA21ならびにMON810系統)の定量PCR法を対象とした外部精度管理試験

概要

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To ensure the reliability of the results obtained by the GMOs analytical methods, analysts must maintain and improve their technical abilities. Moreover, it is very important to reveal the factors affecting the analytical results for technical improvements. In this study, a laboratory-performance study was carried out to investigate factors affecting the analytical results obtained by the quantitative PCR method to analyze the approved genetically modified maize (GA21 and MON810 lines). After examination of the homogeneity and stability of the prepared test samples, the samples were provided to the 33 laboratories participating in this collaborative study. The provided samples consist of two test materials, GA21L (including each GA21 and MON810 line in 1% concentration) and GA21H (including 5% GA21 and 1% MON810 line). The data were collected from all the laboratories and statistically analyzed. In a few laboratories which reported the error test values, the DNA quality was low or the yield was unstable. Furthermore, the value measured for the taxon-specific gene was also unstable. These results suggest that technical improvement may be needed for these laboratories regarding the DNA extraction and/or preparation step of the PCR reagents.
日本食品化学学会の論文
2006-05-08