In Vitro Study for Inhibition of NO Production about Constituents of Sappan Lignum(Pharmacognosy)
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概要
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In the course of our screening, we found that the methanolic extract of Sappan Lignum showed strong activity against lipopolysaccharide (LPS)-induced nitric oxide (NO) production by macrophages in vitro. As it was reported that Brazilin inhibited inducible NO gene, we conducted to similar tests for six known compounds isolated from Sappan Lignum, namely, brazilein, sappanchalcone, protosappanin A, protosappanin B, protosappanin C besides brazilin. And six compounds were also subjected to six tests to speculate their properties: (1) inhibition of NO production by cultured J774.1 (macrophage-like) cell line, (2) suppression of inducible NO synthase (iNOS) gene expression, (3) inhibition of NO production by murine peritoneal macrophages, (4) DPPH radical scavenging activity, (5) reduction of ferric ion and (6) antioxidant activity. Brazilein and sappanchalcone showed significant inhibition of lipopolysaccharide (LPS)-induced NO production by J774.1 cell line like Brazilin; 100% inhibition at 30μM in test (1) and at 10μM in test (3). The mechanisms underlying the inhibition of NO production by the compounds were investigated in test (2). As a result, brazilin was found to almost completely suppress iNOS gene expression at 100μM as reported, and brazilein and sappanchalcone also suppressed iNOS gene expression. But strong activities were not observed for protosappanins A, B and C. So, we conducted tests (4), (5) and (6) to investigate other properties about six compounds. Protosappanin A and Brazilin demonstrated high antioxidant activity compared with Vitamin E in tests (4) and (5). Protosappanin A and B inhibited the oxidation of linoleic acid in test (6). Among the dibenzoxocin derivatives, only protosappanin C did not show significant activity in all the tests. We found that sappanchalcone showed same activity as brazilin, and six compounds isolated from Sappan Lignum showed various properties.
- 公益社団法人日本薬学会の論文
- 2007-01-01
著者
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Sasaki Yohei
Department of Medicinal Plant Science, Hoshi University
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Nagumo Seiji
Department of Medicinal Plant Science, Hoshi University
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Nagumo Seiji
Dep. Of Medicinal Plant Sci. Hoshi Univ.
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Nagumo Seiji
Faculty Of Pharmaceutical Sciences Hoshi University
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Nagai Masahiro
Department Of Medicinal Plant Sciences Hoshi University
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HOSOKAWA Tomokazu
Department of Pharmacology, School of Pharmacy, Hoshi University
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Nagumo Seiji
Dep. Of Medicinal Plant Sciences Hoshi Univ.
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Hosokawa Tomokazu
Dep. Of Medicinal Plant Sciences Hoshi Univ.
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Hosokawa Tomokazu
Department Of Pharmacology Hoshi College Of Pharmacy
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Nagai Msahiro
Department Of Medicinal Plant Sciences Hoshi University
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Sasaki Yohei
Department Of Materials Science And Technology Faculty Of Science And Technology Hirosaki University
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Nagai Masahiro
Department Of Biodesign Division Of Biosystems Institute Of Biomaterials And Bioengineering Tokyo Me
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Sasaki Youhei
Faculty Of Pharmaceutical Sciences Hoshi University
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Nagumo S
Dep. Of Medicinal Plant Sciences Hoshi Univ.
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Sasaki Yohei
Department Of Medicinal Plant Science Hoshi University
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Nagumo Seiji
Department Of Medicinal Plant Science Hoshi University
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Hosokawa Tomokazu
Faculty Of Pharmaceutical Sciences Hoshi University
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Hosokawa Tomokazu
Department of Medicinal Plant Science, Hoshi University
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