Rapid and Sensitive Detection of Lophophora williamsii by Loop-Mediated Isothermal Amplification

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概要

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• We have developed a convenient method for the detection of Lophophora williamsii using loop-mediated isothermal amplification (LAMP). We designed six species-specific primers for L. williamsii, including two loop primers. This L. williamsii-specific primer set was used for LAMP of total DNA extracted from L. williamsii and from L. diffusa. Real-time monitoring of LAMP was achieved by measuring turbidity due to the formation of magnesium pyrophosphate. Amplification occurred in samples mixed with total DNA from L. williamsii, but not in those mixed with total DNA from L. diffusa. We could also visually observe L. williamsii by adding fluorescent detection reagent to the reaction tube and exposing it to UV light. This new method amplified L. williamsii selectively and is expected to be applicable to the detection of peyote.

• 日本薬学会の論文

著者

• 藤本 嗣人

  国立感染症研究所感染症情報センター

• Sasaki Yohei

  Department of Medicinal Plant Science, Hoshi University

• Fujimoto Tsuguto

  National Institute of Infectious Disease

• Aragane Masako

  Medicinal Plant Garden, Tokyo Metropolitan Institute of Public Health

• Yasuda Ichiro

  Laboratory of Public Health, Faculty of Pharmacy, Chiba Institute of Science

• Nagumo Seiji

  Department of Medicinal Plant Science, Hoshi University

• Sasaki Yohei

  Department Of Materials Science And Technology Faculty Of Science And Technology Hirosaki University

• Sasaki Yohei

  Department Of Medicinal Plant Science Hoshi University

• Nagumo Seiji

  Department Of Medicinal Plant Science Hoshi University

• Aragane Masako

  Medicinal Plant Garden Tokyo Metropolitan Institute Of Public Health

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• 緒言

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