茶カテキン類の効率的抽出と利用に関する研究 : 1)活性白土によるcaffeine除去とカテキンカルシウム塩の調製

概要

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Catechins [(-)-epigallocatechin (EGC), (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin 3-O-gallate (EGCG), (-)-epicatechin 3-O-gallate (ECG)] in tea leaf are important natural phenolic materials used in various industries. In this experiment, catechins were easily isolated from water extract of tea leaf by simple procedures as follows: 1) removal of caffeine by the addition of clay which mainly adsorb caffeine and not catechins 2) mass precipitation of catechin- calcium salt by the addition of calcium hydroxide. Catechin- calcium salt obtained as above contained 36.3% of EGCG, 7.3% of EGC, 1.7% of C, 2.0% of EC and 3.9% of ECG (as dry weight) with approximately 10% of calcium. The procedure (preparation of catechin- calcium salt) is especially good for the isolation of EGCG and safety without using of any organic solvents.
2001-03-31