Blackstonia perfoliataおよび3種のGentiana属植物の組織培養と二次代謝成分

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Shoot and callus cultures of Blackstonia perfoliata and three Gentiana species, G. lutea, G. triflora and G. rubicunda, were established and the amounts of bitter principles such as swertiamarin, gentiopicrin, 2'-(o, m-dihydroxybenzoyl) sweroside and amarogentin and phenolics such as apigenin and gentisin in the tissues were determined. B. perfoliata plantlets, in vitro cultured on phytohormone-free 1/2 MS medium, accumulated a large amount of gentiopicrin (maximum: 4.49 % dry weight in leaf portion), the level of which was comparable to that of G. lutea. B. perfoliata callus, cultured on 1/2 MS medium supplemented with 3.0 mg/l IAA, also produced a large amount (approximately 1.4 % dry weight) of gentiopicrin both in the light and dark conditions, whereas the calli of G. lutea, G. triflora and G. rubicunda produced scarcely any amounts of bitter principles and phenolics.
1998-12-20